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Empagliflozin attenuates Cyclophosphamide-induced hepatotoxicity in male rats
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Izadpanah Gheitasi   1, Hosein Sadeghi2 , Ismaeel Panahi Kokhdan2 , Ghaidafe Akbari2 , Mahdokht Azizi2 , Elaheh Azargoun2 |
1- yasuj university of medical sciences , gheitasiizadpanah@yahoo.com
2- yasuj university of medical sciences |
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Abstract: (198 Views) |
Introduction: Cyclophosphamide(CP) is a medication primarily used in the management and treatment of neoplasms, including lymphoma, Leukemia, and breast cancer . This drug also has significant immunosuppressive activity . despite its wide clinical use, CP also possesses many adverse effects, including hepatotoxicity that leads to mitochondrial oxidative stress , hepatic necrosis and cell death. Empagliflozin(EMPA) is a Sodium-glucose cotransporter-2 inhibitors that used to treat diabetes and have antioxidant activity . This study was designed to investigate the effect of Empagliflozin on hepatotoxicity induced by cyclophosphamide in male rats.
Materials and Methods: In this experimental study, 24 male Wistar rats were divided into four groups: control group, CP group, EMPA+CP group and CP+EMPA group. All groups were treated for 11 days . blood samples were obtained and the liver was removed . plasma levels of ALT, AST and ALP were measured . Homogenized liver tissue was used to measure malondialdehyde (MDA), Nitric Oxide (NO). Liver histology was also performed . The results were analyzed by SPSS , all data are expressed as means ± standard errors of the mean and differences with p < 0.05 were considered statistically significant.
Results: The results showed that there was a significant increase in ALT , AST, NO metabolites and MDA in the cyclophosphamide group when compared with control group. Changes in ALP enzyme between groups were not significant. Empagliflozin led to a significant decrease in plasma ALT and AST enzymes also tissue NO and MDA in in comparison with CP group . Based on the pathology results, the toxic effect of cyclophosphamide was proved through the increase of inflammation, edema , congestion and necrosis in the liver tissue that Empagliflozin reduced these toxic effects.
Conclusion: According to the results of this study, Empagliflozin can reduce the hepatotoxicity of cyclophosphamide with its antioxidant effect.
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| Keywords: Cyclophosphamide, Empagliflozin, Liver Enzymes, Malondialdehyde |
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Type of Study: Research |
Subject:
Physiology
Received: 2023/10/10 | Accepted: 2023/12/26 | Published: 2024/03/16
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