|
The effect of combined exercise and curcumin supplementation on TCF-1 and GSK3β gene expression in the brains of rats with glioblastoma multiforme
|
Yaser Kazemzadeh1   , Samaneh Afsharyousefi2 , Hossein Shirvani3 , Sanaz Mirzayan Shanjani 4 |
1- Department of Sports Physiology, Islamshahr Branch, Islamic Azad University, Islamshahr, Iran
2- PhD in Sports Physiology, Islamshahr Branch, Islamic Azad University, Islamshahr, Iran
3- Sports Physiology Research Center, Lifestyle Research Institute, Baqiyat Elah University of Medical Sciences (AJ), Tehran, Iran.
4- Department of Sports Physiology, Islamshahr Branch, Islamic Azad University, Islamshahr, Iran , san_mir2000@yahoo.com |
|
|
Abstract: (327 Views) |
Introduction: lifestyle modification and the use of nanotechnology are among the treatment methods for cancers. The purpose of this study was to investigate the effect of combined exercise and curcumin supplementation on T-cell factor 1 (TCF-1) and Glycogen synthase kinase 3 beta (GSK3β) gene expression in the brain of rat with glioblastoma multiforme.
Methods: In this experimental study, 40 male Wistar rats were divided into 5 healthy control groups, glioblastoma multiforme (GBM), GBM+ combined training (CT), GBM+ nanocurcumin supplement (N-CUR) and GBM+CT+N-CUR (8 rats in each group). Glioblastoma was injected into the frontal cortex of rat. Exercise training was performed for 4 weeks and 3 days per week as a combination of aerobic and resistance training. Nano curcumin supplement was gavage with a dose of 80 mg/kg for 4 weeks, 5 days per week. At the end, rats sacrificed and TCF-1 and GSK3β genes of brain glioblastoma were analyzed by Real-time PCR method. Shapiro-Wilk test was used to determine the normality of the data.
Results: The results of the present study showed that the expression of TCF1 mRNA and GSK3β mRNA in the brain tumor tissue of rats showed a significant increase compared to the healthy control group (p<0.0001 for both). However, compared to the GBM group, all the treatment groups, especially the GBM+CT+N-CUR group, showed a significant decrease in the expression of TCF1 mRNA and GSK3β mRNA in the brain tumor tissue (p<0.05). The correlation between these two factors was not confirmed in different research groups (p>0.05).
Conclusion: It seems that the combination of exercise and Nano curcumin supplement be an effective treatment method in down regulation of some effective genes in brain tumor tissue. However, in this field, more studies are needed, especially in human samples. |
|
| Keywords: Combined exercise, Brain tissue, Glioblastoma, Curcumin |
|
|
|
Type of Study: Research |
Subject:
Sport Physiology
Received: 2024/01/26 | Accepted: 2024/09/9 | Published: 2024/10/6
|
|
|
|
|
|
|
| Send email to the article author |
|
|
|
| Add your comments about this article |
|
|
|
