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:: Volume 27, Issue 5 (8-2022) ::
__Armaghane Danesh__ 2022, 27(5): 0-0 Back to browse issues page
Investigating histological changes and gene expression of interleukin-8, interleukin-12, and interferon beta in liver tissue after consumption of Lactobacillus rhamnosus GG probiotic in steatosis model rats
Fatemeh Rostamkhani
Department of Biology, Yadegar-e-Imam Khomeini (RAH) Shahre Rey Branch, Islamic Azad University, Tehran, Iran. , Shirinrostamkhani@yahoo.com
Abstract:   (43 Views)
Abstract
Background and purpose: Probiotic consumption is able to regulate the homeostasis of the digestive system and control metabolic diseases related to the digestive system. Therefore, the purpose of this study is to investigate histological changes and gene expression of interleukin 12, 8 and interferon beta in liver tissue after 5 weeks of consumption of probiotic (Lactobacillus rhamnosus Gorbach-Goldin) in steatosis model rats.
Methods: 32 male Wistar rats were randomly divided into 4 groups (each group 8 heads): control (CON), steatosis (ST), probiotic (PRO) and steatosis + probiotic (ST+PRO). Rats were modeled by intraperitoneal injection of 140 mg/kg tetracycline (an antibiotic known to cause steatosis). The amount of 107 colony forming units (cfu) of LGG were considered for probiotic groups for 5 weeks (5 days per week, by gavage). Hematoxylin-eosin staining was used to examine histological changes. Also, changes in IL-12, IL-8 and IFN-β gene expression were used using RT-PCR method. The data were analyzed by one-way analysis of variance.
Results: Liver tissue histology improved with 5 weeks of probiotic consumption after ST model. ST group showed a significant increase in IL-12, IL-8 and IFN-β gene expression (p<0.05). Compared to the ST group, the PRO and ST+PRO groups showed a significant decrease in IL-12, IL-8 and IFN-β gene expression (p<0.05).
Conclusion: It seems that the consumption of probiotics is able to improve the cellular and tissue of the liver in people with fatty liver problems by negatively regulating some cellular risk factors. However, more studies are needed, especially on human samples.
 
Keywords: Hepatic Steatosis, Probiotic, Pro- and Anti-inflammatory Cytokines, Interferon-β
     
Type of Study: Research | Subject: Physiology
Received: 2022/07/26 | Accepted: 2022/09/12 | Published: 2022/09/13
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Rostamkhani F. Investigating histological changes and gene expression of interleukin-8, interleukin-12, and interferon beta in liver tissue after consumption of Lactobacillus rhamnosus GG probiotic in steatosis model rats. ارمغان دانش. 2022; 27 (5)
URL: http://armaghanj.yums.ac.ir/article-1-3346-en.html


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Volume 27, Issue 5 (8-2022) Back to browse issues page
ارمغان دانش Armaghane Danesh
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