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The Effect of Morphine on the Final Stages of Oocyte Meiosis in Culture and Fertilization Media in Mice
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SN Nemat Elahi Mahani1    , S Amini 2, F Nabipour3 , SH Iftikhar Vaqefi1 |
1- Department of Anatomy, Kerman University of Medical Sciences, Kerman, Iran
2- Department of Anatomy, Kurdistan University of Medical Sciences, Kurdistan, Iran , sh_amini876@yahoo.com
3- Department of Pathology, Kerman University of Medical Sciences, Kerman, Iran |
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Abstract: (1829 Views) |
Background & aim: Addictive substances by disturbing the hypothalamic-pituitary-ovarian axis can affect the reproductive system and affect the secretion of gonadotropins. Morphine significantly reduces plasma LH levels. This poses a serious threat to fertility and pregnancy by causing abnormal changes in the cell structure and endometrial tissue. Therefore, the aim of the present study was to investigate the effect of morphine consumption on the final stages of oocyte meiosis in culture and in vitro fertilization in mice.
Methods: In the present experimental study conducted at Kerman University of Medical Sciences in 2013, sixteen female mice weighing an average of 20-24 g were divided into treatment and control groups. During three weeks, the treatment group had free access to water impregnated with morphine sulfate (0.4 mg / ml). To stimulate ovulation, serum gonadotropin was injected into pregnant mares and the animals were sacrificed after 48 hours. The cumulus-euphorus complex was extracted from the ovaries and transferred to the enriched medium. After 24 hours, the number of oocytes containing the first polar body (metaphase II egg) was recorded and transferred to the fertilization medium. Sperms were extracted from vas deferens of male mice and after preliminary steps such as centrifugation and incubation, they were transferred to the fertilization medium under appropriate conditions. Data were analyzed using t-test and chi-square statistical tests.
Results: The mean ovarian weight of the control and treatment groups were 6.7 and 7.3 mg, respectively (p=0.7) in the control group out of 128 metaphase I eggs, 89 metaphase II eggs (69.53%) and in treatment group out of 140 metaphase I, 99 metaphase II oocytes (70.71%) were extracted (p = 0.051). The control group had 29 cases of positive fertilization out of 89 metaphase II eggs (32.57%) and the treatment group had 31 positive fertilization cases (31.31%) out of 99 metaphase II eggs (p = 0.21).
Conclusion: It seems that despite the dependence of the animal, morphine did not interfere with the resumption and completion of meiosis and thus the ability of the egg to reproduce in the culture medium was not impaired.
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| Keywords: Morphine, Meiosis, Oocyte, Fertility, Mice |
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Full-Text [PDF 1494 kb]
(475 Downloads)
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Type of Study: Research |
Subject:
Anatomy
Received: 2020/02/18 | Accepted: 2020/09/30 | Published: 2020/11/7
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