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:: Volume 25, Issue 5 (10-2020) ::
__Armaghane Danesh__ 2020, 25(5): 0-0 Back to browse issues page
The effect of chronic administration of morphine on end stages of oocyte meiosis and in vitro fertilization in Syrian mice
Norreddin Nematollahi mahani Seyyed1 , Sarah Amini 2, Hasan Eftekharvaghefi Seyyed1 , Fateme Nabipour Seyyede1
1- kerman medical university
2- kurdistan medical university , sh_amini876@yahoo.com
Abstract:   (104 Views)

substances can effect on the Hypothalamus-hypophysis-ovary and affect the performance of the
Addictive substances can affect the reproductive system and they affect the secretion of
gonadotropins. Morphine significantly reduces plasma LH levels in all range groups of men and women.
Most illicit  drugs and opioids, including morphine, are associated with decreased physical stimulation,
 decreased libido, decreased ability to reach orgasm (in women), and delayed or stopped ejaculation in
men. Morphine can pose a serious threat to fertility and pregnancy by causing abnormal changes in the
cell structure and lining of the endometrium. In this study, due to the common use of morphine as a
narcotic that leads to addiction and the lack of information on the effect of morphine on ovum in culture
medium, the effect of morphine on cell development and its fertility in culture medium has been

Material and methods

Sixteen female mice with average weight 20-24gm divided into control and treated groups. During three
 weeks, treated group got access water infected with 0/4 mg/ml morphine sulfate. In order to
superovulation, pregnant mare serum gonadotropin injected. Animals sacrificed and ovaries removed.
The cumulus-Oocyte complex from the ovaries transferred to medium. After 24 hours, Oocytes
containing the first polar body was transferred to in vitro fertilization medium.  Male mice was sacrificed
and spermatozoids from vas deferens stored in incubator for 2 hours, and then sperms transferred into
IVF medium. Finally, the Oocytes observed for second polar body or two cell.

Results:The mean ovarian weight of the control and treatment group was 6.95 mg and 7.2 mg
respectively that was not significant. In control group out of 128 metaphase I Ooctytes, 89 metaphase II
ovum (69.53%) and in treatment group 99 metaphase II ovum were obtained from 140 metaphase I
 Oocytes (70.71%) (p = 0.051). Also in control group 29 case positive fertilization from 89 MII
Oocytes(32.58%) and in treatment group 31 cases positive fertilization from 99 MII Oocytes(31.31%) was

Conclusion: It seems that despite its dependence on the animal, morphine does not interfere with the
 resumption and completion of meiotic division and thus does not impair egg fertility in the culture
Key words
Morphine،miosis,،Oocyte Fertility،Mice
Keywords: Morphine, Maturation, Fertility, meiosis, Invitro, Mice
Type of Study: Research | Subject: Anatomy
Received: 2020/02/18 | Accepted: 2020/09/30 | Published: 2020/11/7
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nematollahi mahani N, amini S, eftekharvaghefi H, nabipour F. The effect of chronic administration of morphine on end stages of oocyte meiosis and in vitro fertilization in Syrian mice. Armaghane danesh. 2020; 25 (5)
URL: http://armaghanj.yums.ac.ir/article-1-2728-en.html

Volume 25, Issue 5 (10-2020) Back to browse issues page
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