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The Influence of Iontophoresis on Tretinoin Release from Topical Preparations and Its Penetration across Rat Skin
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M Heshmatipour   , N Tavakoli 1, R Ebrahimi |
| 1- , tavakoli@pharm.mui.ac.ir |
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Abstract: (5219 Views) |
| ABSTRACT:
Introduction & Objective: Acne vulgaris is one of the most
prevalent skin diseases which is common in adolescent of both
sexes. Tretinoin has been used therapeutically for its comedolitic
effects and inhibition of comedone rupture in acne. It is available
in 0.01% to 0.1% as cream, gel or lotion. There have been a few
clinical reports of the positive effects of iontophoresis with
tretinoin on acne scars. The aim of this study was to investigate
the effect of iontophoresis field on transport of tretinoin across
synthetic membrane and excised rat skin.
Materials & Methods: Topical formulations of tretinoin marketed
in Iran ( Retin-A cream, Retin-A gel, Tretinoin cream 0.025% and
Tretinoin gel 0.025%) were assessed in terms of drug assay and
content uniformity. The in vitro release and penetration
characteristics of tretinoin from dermatological preparation was
studied through a hydrophilic Dora pore diffusion barriers and
membrane excised rat skin using Franz diffusion cell over a
period of 2h. These two tests were also accomplished either with
or without the presence of an iontophoresis field. The current
density of iontophoresis applied field was constant, 0.5 mA/cm2.
The amount of drug released from preparations was determined
spectrophotometrically at max =352nm.
Results: The in vitro release of tretinoin through synthetic barrier
with an iontophoresis field showed that the cumulative percent of
released drug at the end of each experiment was 17.5%, 26.5%,
19% and 54% for Tretinoin gel, Retin-A gel, Tretinoin cream and
Retin-A cream, respectively. This was significantly higher than
the observed one for release of tretinoin without application of a
DC electric field. In addition, the results showed a significantly
higher iontophoretic transport of tretinoin compared to passive
flux for excised rat skin.
Conclusion: The application of an electric current for a limited
period of time produced higher post-iontophoresis fluxes of
tretinoin than passive diffusion. With application of iontophoresis
field, the in vitro release and transport of drug for different
formulations of tretinoin follow the zero order kinetics. |
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| Keywords: Iontophoresis, Rat skin, Tretinoin |
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Full-Text [PDF 173 kb]
(1146 Downloads)
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Type of Study: Research |
Subject:
Special
Received: 2015/06/15 | Accepted: 2015/06/15 | Published: 2015/06/15
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