|
Investigating the prevalence of Clostridium perfringens and Bacillus cereus contamination in sandwiches sold in Khorramabad city and determining the antibiotic resistance of the isolates
|
Fatemeh Ghasemi1   , Najmeh Vahed dehkordi2 , Seyed majid Hashemi1 |
1- Azad uni
2- Azad uni , najmeh.vaheddehkordi@iau.ir |
|
|
Abstract: (5 Views) |
Background and aim: Bacillus cereus and Clostridium perfringens are spore-forming bacteria that cause food spoilage and vomiting and diarrhea syndromes in humans. The aim of the present study was to investigate the prevalence of contamination with Clostridium perfringens and Bacillus cereus in sandwiches sold in Khorramabad city and the antibiotic resistance of the isolates.
Methods: 100 food samples were randomly sampled from the market in Khorramabad city and transferred to the laboratory. The number of positive samples along with the Clostridium perfringens cpe gene and nheB and hblA in Bacillus cereus were determined, and then the antibiotic resistance of the isolates was determined. The chi-square (χ2) test was used to compare the frequencies.
Results: The results showed that out of 100 food samples, 9% were contaminated with Bacillus cereus and 16% with Clostridium perfringens. PCR results showed that 37.5% of the isolates carried the cpe gene, 55.6% with the nheA gene, and 33.3% with the hblA gene. Statistical evaluations showed that there was no significant relationship between contamination with Bacillus cereus and Clostridium perfringens (p>0.05). Antibiotic resistance results showed that the highest resistance in Clostridium perfringens was related to tetracycline (32.2%) and in Bacillus cereus to penicillin (100%).
Conclusion: Discussion and Conclusion: The results showed that street foods have a high potential for contamination with Clostridium perfringens and Bacillus cereus, which requires the competent authorities to enact strict laws. Also, in case of poisoning with the aforementioned bacteria, the arbitrary use of antibiotics should be avoided.
|
|
| Keywords: Food safety, Bacillus cereus, Clostridium perfringens, Khorramabad |
|
|
|
Type of Study: Research |
Subject:
Microbiology
Received: 2025/12/16 | Accepted: 2026/04/5 | Published: 2026/05/23
|
|
|
|
|
|
|
| Send email to the article author |
|
|
|
| Add your comments about this article |
|
|
|
