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The effect of dexmedetomidine on the oxidative stress status of kidney tissue following chronic restraint stress in male mice
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Seyede Mahdieh Ghoreyshi1   , Mohammadreza Nasirzadeh2 , Aref Delkhoush3 |
1- Tabriz medical sciences, Islamic Azad University, Tabriz, Iran.
2- Tabriz medical sciences, Islamic Azad University, Tabriz, Iran. , mr.nasirzadeh@iau.ir
3- Faculty of Veterinary Medicine, Urmia University, Urmia, Iran. |
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Abstract: (4 Views) |
Introduction and Objective: The increasing use of tobacco in human societies has led to widespread diseases caused by nicotine intake. Therefore, the aim of the present study was to investigate the effect of different doses of nicotine on antioxidant function, oxidative stress, and histopathological changes in the liver in male rats.
Methods: In this experimental laboratory study, 24 male Wistar rats were randomly divided into 3 groups (n= 8) including 1)control (daily intraperitoneal injection of normal saline),2) nicotine 0.25 mg/kg intraperitoneally daily, and3) nicotine 0.5 mg/kg intraperitoneally for 30 days.
At the end of the study period, the activities of antioxidant enzymes superoxide dismutase (SOD), glutathione peroxidase (GPX), total antioxidant capacity (TAC), and malondialdehyde (MDA) levels in liver tissue, as well as serum ALT and AST enzymes, were measured.
Results: The results of the study showed that nicotine administration caused a significant increase in ALT, AST and MDA levels and a decrease in serum SOD activity (p<0.05). Also, a significant decrease in SOD and GPX enzyme activities and TAC levels and an increase in liver tissue MDA levels were reported (p<0.05).
In addition, the results of liver histology changes showed that nicotine use caused congestion and hyperemia, lymphocyte infiltration and inflammation, cell degeneration, and necrosis.
Conclusion: This study showed that nicotine administration, through the induction of oxidative stress, leads to an increase in serum and liver tissue malondialdehyde levels and causes tissue changes and liver damage by reducing the activity of antioxidant enzymes.
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| Keywords: Oxidative stress, nicotine, liver, rat. |
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Type of Study: Research |
Subject:
Physiology
Received: 2025/07/12 | Accepted: 2026/02/8 | Published: 2026/05/23
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