fa بررسی اثرات وابسته به دوز نیکوتین بر استرس اکسیداتیو و هیستوپاتولوژی کبد در موش صحرایی نر فیزیولوژی Physiology پژوهشي Research <span style="font-size:11pt"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="line-height:107%"><span sans-serif="" style="font-family:Calibri,"><b><span lang="FA" style="font-family:Yagut">مقدمه وهدف</span></b>:<span lang="FA" style="font-family:Yagut">افزایش روز افزون مصرف دخانیات در جوامع انسانی موجب بروز بیماری های گسترده ناشی از دریافت نیکوتین شده است. لذا هدف از انجام مطالعه حاضر بررسی تاثیر دوز های مختلف نیکوتین بر عملکرد آنتی اکسیدانی،استرس اکسیداتیو و تغییرات هستوپاتولوژیک کبد در موش های صحرایی نر بود.</span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="line-height:107%"><span sans-serif="" style="font-family:Calibri,"><b><span lang="FA" style="font-family:Yagut">روش ها:</span></b><span lang="FA" style="font-family:Yagut">در این مطالعه تجربی آزمایشگاهی، تعداد 24 سر موش صحرائی نر نژاد ویستار به صورت تصادفی به 3 گروه 8 تایی شامل 1)کنترل( تزریق روزانه داخل صفاقی نرمال سالین)،2)گروه نیکوتین 25/0 و 3)گروه نیکوتین 5/0(تزریق نیکوتین با دوز 25/0و5/0بصورت داخل صفاقی ) به مدت 30 روز تقسیم بندی شدند.</span> <span lang="FA" style="font-family:Yagut">در پایان دوره مطالعه، فعالیت آنزیم&shy;های آنتی&shy;اکسیدان سوپراکسید دسموتاز </span>(SOD)<span style="font-family:Yagut"> گلوتاتیون پراکسیداز </span>(GPX)<span lang="FA" style="font-family:Yagut"> ظرفیت تام آنتی&shy;اکسیدانی </span>(TAC)<span lang="FA" style="font-family:Yagut"> و سطح مالون دی آلدهید </span>(MDA)<span lang="FA" style="font-family:Yagut"> بافت کبدو آنزیم های </span>ALT<span lang="FA" style="font-family:Yagut"> و</span>AST<span lang="FA" style="font-family:Yagut"> سرم اندازه گیری شدند.</span><span lang="FA" style="font-family:Yagut"></span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span style="direction:rtl"><span style="unicode-bidi:embed"><span sans-serif="" style="font-family:Calibri,"><b><span lang="FA" style="font-family:Yagut">یافته ها:</span></b><span lang="FA" style="font-family:Yagut">نتایج مطالعه نشان داد که تجویز نیکوتین موجب افزایش معنی دار آنزیم های</span><span dir="LTR" new="" roman="" style="font-family:" times="">ALT</span><span style="font-family:Yagut"> ، </span><span dir="LTR" new="" roman="" style="font-family:" times="">AST</span><span style="font-family:Yagut"> &nbsp;و سطح</span><span dir="LTR" new="" roman="" style="font-family:" times="">MDA</span><span lang="FA" style="font-family:Yagut"> و کاهش فعالیت</span><span dir="LTR" new="" roman="" style="font-family:" times="">SOD</span><span style="font-family:Yagut"> سرم گردید(05/0></span><span dir="LTR" new="" roman="" style="font-family:" times="">p</span><span lang="FA" style="font-family:Yagut">). همچنین کاهش معنی دارفعالیت آنزیم های </span><span dir="LTR" new="" roman="" style="font-family:" times="">SOD</span><span lang="FA" style="font-family:Yagut">،</span><span dir="LTR" new="" roman="" style="font-family:" times="">GPX</span><span lang="FA" style="font-family:Yagut"> و میزان</span><span dir="LTR" new="" roman="" style="font-family:" times="">TAC</span><span lang="FA" style="font-family:Yagut"> و افزایش سطح </span><span dir="LTR" new="" roman="" style="font-family:" times="">MDA</span><span style="font-family:Yagut"> بافت کبد گزارش گردید(05/0></span><span dir="LTR" new="" roman="" style="font-family:" times="">p</span><span lang="FA" style="font-family:Yagut">). علاوه بر این، نتایج هیستولوژی کبد نشان داد که استعمال نیکوتین موجب احتقان و پرخونی،اینفیلتراسیون سلول های لنفاوی و التهاب، دژنرسانس سلولی و نکروز شده است.</span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span style="direction:rtl"><span style="unicode-bidi:embed"><span sans-serif="" style="font-family:Calibri,"><b><span lang="FA" style="font-family:Yagut">نتیجه گیری: </span></b><span lang="FA" style="font-family:Yagut">این مطالعه نشان داد که تجویز نیکوتین از طریق القا استرس اکسیداتیو منجر به افزایش سطح مالون دی آلدئید سرم و بافت کبد شده و با کاهش فعالیت آنزیم های آنتی اکسیدانی موجب تغییرات بافتی و آسیب کبدی می شود.</span></span></span></span></span></span><br> &nbsp; <span style="font-size:11pt"><span style="background:#f8f9fa"><span style="line-height:27.0pt"><span sans-serif="" style="font-family:Calibri,"><b><span lang="EN" style="font-size:10.0pt"><span arial="" style="font-family:"><span style="color:#1f1f1f">Introduction and Objective</span></span></span></b><span lang="EN" style="font-size:10.0pt"><span arial="" style="font-family:"><span style="color:#1f1f1f">: The increasing use of tobacco in human societies has led to widespread diseases caused by nicotine intake. Therefore, the aim of the present study was to investigate the effect of different doses of nicotine on antioxidant function, oxidative stress, and histopathological changes in the liver in male rats.</span></span></span><span style="font-size:10.0pt"><span arial="" style="font-family:"><span style="color:#1f1f1f"></span></span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="background:#f8f9fa"><span style="line-height:27.0pt"><span style="tab-stops:45.8pt 91.6pt 137.4pt 183.2pt 229.0pt 274.8pt 320.6pt 366.4pt 412.2pt 458.0pt 503.8pt 549.6pt 595.4pt 641.2pt 687.0pt 732.8pt"><span sans-serif="" style="font-family:Calibri,"><b><span lang="EN" style="font-size:10.0pt"><span arial="" style="font-family:"><span style="color:#1f1f1f">Methods</span></span></span></b><span lang="EN" style="font-size:10.0pt"><span arial="" style="font-family:"><span style="color:#1f1f1f">: In this experimental laboratory study, 24 male Wistar rats were randomly divided into 3 groups (n= 8) including 1)control (daily intraperitoneal injection of normal saline),2) nicotine 0.25 mg/kg intraperitoneally daily, and3) nicotine 0.5 mg/kg intraperitoneally for 30 days.</span></span></span><span style="font-size:10.0pt"><span arial="" style="font-family:"><span style="color:#1f1f1f"></span></span></span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="background:#f8f9fa"><span style="line-height:27.0pt"><span style="tab-stops:45.8pt 91.6pt 137.4pt 183.2pt 229.0pt 274.8pt 320.6pt 366.4pt 412.2pt 458.0pt 503.8pt 549.6pt 595.4pt 641.2pt 687.0pt 732.8pt"><span sans-serif="" style="font-family:Calibri,"><span lang="EN" style="font-size:10.0pt"><span arial="" style="font-family:"><span style="color:#1f1f1f">At the end of the study period, the activities of antioxidant enzymes superoxide dismutase (SOD), glutathione peroxidase (GPX), total antioxidant capacity (TAC), and malondialdehyde (MDA) levels in liver tissue, as well as serum ALT and AST enzymes, were measured.</span></span></span><span style="font-size:10.0pt"><span arial="" style="font-family:"><span style="color:#1f1f1f"></span></span></span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="background:#f8f9fa"><span style="line-height:27.0pt"><span style="tab-stops:45.8pt 91.6pt 137.4pt 183.2pt 229.0pt 274.8pt 320.6pt 366.4pt 412.2pt 458.0pt 503.8pt 549.6pt 595.4pt 641.2pt 687.0pt 732.8pt"><span sans-serif="" style="font-family:Calibri,"><b><span lang="EN" style="font-size:10.0pt"><span arial="" style="font-family:"><span style="color:#1f1f1f">Results</span></span></span></b><span lang="EN" style="font-size:10.0pt"><span arial="" style="font-family:"><span style="color:#1f1f1f">: The results of the study showed that nicotine administration caused a significant increase in ALT, AST and MDA levels and a decrease in serum SOD activity (p<0.05). Also, a significant decrease in SOD and GPX enzyme activities and TAC levels and an increase in liver tissue MDA levels were reported (p<0.05).</span></span></span><span style="font-size:10.0pt"><span arial="" style="font-family:"><span style="color:#1f1f1f"></span></span></span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="background:#f8f9fa"><span style="line-height:27.0pt"><span style="tab-stops:45.8pt 91.6pt 137.4pt 183.2pt 229.0pt 274.8pt 320.6pt 366.4pt 412.2pt 458.0pt 503.8pt 549.6pt 595.4pt 641.2pt 687.0pt 732.8pt"><span sans-serif="" style="font-family:Calibri,"><span lang="EN" style="font-size:10.0pt"><span arial="" style="font-family:"><span style="color:#1f1f1f">In addition, the results of liver histology changes showed that nicotine use caused congestion and hyperemia, lymphocyte infiltration and inflammation, cell degeneration, and necrosis.</span></span></span><span style="font-size:10.0pt"><span arial="" style="font-family:"><span style="color:#1f1f1f"></span></span></span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="background:#f8f9fa"><span style="line-height:27.0pt"><span style="tab-stops:45.8pt 91.6pt 137.4pt 183.2pt 229.0pt 274.8pt 320.6pt 366.4pt 412.2pt 458.0pt 503.8pt 549.6pt 595.4pt 641.2pt 687.0pt 732.8pt"><span sans-serif="" style="font-family:Calibri,"><b><span lang="EN" style="font-size:10.0pt"><span arial="" style="font-family:"><span style="color:#1f1f1f">Conclusion</span></span></span></b><span lang="EN" style="font-size:10.0pt"><span arial="" style="font-family:"><span style="color:#1f1f1f">: This study showed that nicotine administration, through the induction of oxidative stress, leads to an increase in serum and liver tissue malondialdehyde levels and causes tissue changes and liver damage by reducing the activity of antioxidant enzymes.</span></span></span><span style="font-size:10.0pt"><span arial="" style="font-family:"><span style="color:#1f1f1f"></span></span></span></span></span></span></span></span><br> &nbsp; استرس اکسیداتیو، نیکوتین، کبد ، موش صحرایی. Oxidative stress, nicotine, liver, rat. 0 0 http://armaghanj.yums.ac.ir/browse.php?a_code=A-10-3159-1&slc_lang=fa&sid=1 Seyede Mahdieh ghoreyshi سیده مهدیه قریشی ghoreyshi1376@gmail.com 0020438362 100319475328460033039 No Tabriz medical sciences, Islamic Azad University, Tabriz, Iran. دانشگاه آزاداسلامی، علوم پزشکی تبریز، ایران. Mohammadreza Nasirzadeh محمدرضا نصیرزاده mr.nasirzadeh@iau.ir 1380133742 100319475328460033040 Yes Tabriz medical sciences, Islamic Azad University, Tabriz, Iran. دانشگاه آزاداسلامی، علوم پزشکی تبریز، ایران. aref Delkhoush عارف دلخوش arefdelkhosh@yahoo.com 2820067182 100319475328460033041 No Faculty of Veterinary Medicine, Urmia University, Urmia, Iran. ،گروه پاتوبیولوژی،دانشکده دامپزشکی،دانشگاه ارومیه،ارومیه،ایران