The Effect of Eight Weeks of Progressive Aerobic Training and Fisetin Supplementation on TGFβ and FGF2 Gene Expression in Aged Mice with Alzheimer's Disease

Jalali Dehkordi,  A; Azamian Jazi,  A; Jalali Dehkordi , Kh

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Volume 30, Issue 2 (2-2025)

__Armaghane Danesh__ 2025, 30(2): 136-149

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The Effect of Eight Weeks of Progressive Aerobic Training and Fisetin Supplementation on TGFβ and FGF2 Gene Expression in Aged Mice with Alzheimer's Disease

A Jalali Dehkordi¹

, A Azamian Jazi²

, Kh Jalali Dehkordi³

1- Department of Sport Sciences, Shahrekord University, Shahrekord, Iran
2- Department of Sport Sciences, Shahrekord University, Shahrekord, Iran , azamianakbar@yahoo.com
3- Department of Sport Physiology, Isfahan(Khorasgan) Branch, Islamic Azad University, Isfahan, Iran

Abstract: (1889 Views)

Background & aim: TGFβ and FGF2 are important molecular markers for cerebral angiogenesis, which are significantly reduced in Alzheimer's disease (AD) in brain tissue and may be affected by exercise training. Therefore, the present study aimed to investigate the effect of eight weeks of progressive aerobic training and fisetin supplementation on TGFβ and FGF2 gene expression in aged mice with AD.

Methods: In the present experimental study conducted in 2023, thirty healthy elderly male C57BL/6 mice with Alzheimer's disease (AD) were equally divided into five groups, including: healthy control, Alzheimer's (AD), AD + Fisetin, AD + Aerobic Training (AT), and AD + AT + Fisetin. Rats of AD groups developed AD by injecting amyloid beta (Aβ1-42) into the hippocampus. Aerobic training protocol started five days a week for eight weeks with zero slope and a speed of 10 meters per minute for 30 minutes and reached 27 meters per minute for 40 minutes in the eighth week. A fisetin supplement, with a concentration of 20 mg/kg, was used for eight weeks. Data analysis was performed using a one-way analysis of variance and Tukey's post hoc test (P≥0.05).

Results: The changes in the expression of TGF-β and FGF2 genes in the brain tissue of aged mice were significantly different among the five groups (P<0.01), and also based on the results of the post hoc test, their expression in the AD groups decreased significantly (P<0.01) compared to the healthy control group. Also, their expression increased significantly in AD + exercise + fisetin, AD + exercise, and AD + fisetin groups compared to AD group (P<0.01). In addition, their expression in the AD + exercise + fisetin group was significantly higher than the AD + exercise and also their expression was significantly higher in the AD + exercise group than in the AD + fisetin group (P<0.01).

Conclusion: It seems that the combination of progressive aerobic training and fisetin supplement, or each of them alone, can help to increase cerebral angiogenesis in patients with Alzheimer's disease, maybe through increasing the expression of TGFβ and FGF2. Of course, the combination of fisetin and aerobic training has a more significant effect than aerobic training and supplementation alone, and aerobic training is more effective than taking fisetin alone. Therefore, the combination of progressive aerobic training and fisetin is recommended as the most effective complementary treatment method for this disease.

Keywords: Exercise, Angiogenesis, Dietary Supplements, Alzheimer Disease

Full-Text [PDF 648 kb] (20 Downloads)

Type of Study: Research | Subject: Sport Physiology
Received: 2024/07/30 | Accepted: 2024/09/29 | Published: 2025/02/4

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Jalali Dehkordi A, Azamian Jazi A, Jalali Dehkordi K. The Effect of Eight Weeks of Progressive Aerobic Training and Fisetin Supplementation on TGFβ and FGF2 Gene Expression in Aged Mice with Alzheimer's Disease. armaghanj 2025; 30 (2) :136-149
URL: http://armaghanj.yums.ac.ir/article-1-3661-en.html

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