[Home ] [Archive]   [ فارسی ]  
:: Main :: About :: Current Issue :: Archive :: Search :: Submit :: Contact ::
:: Volume 25, Issue 3 (6-2020) ::
__Armaghane Danesh__ 2020, 25(3): 0-0 Back to browse issues page
Hydro-alcoholic extract of Dorema aucheri loaded on liposome nanoparticles, effectively induces apoptosis in SW48 cell line
Ghasem Ghalamfarsa Mr 1, Farzaneh Rezaeinejad Miss2 , Zahra Ghasemifard Ms2 , Maryam Tajali Ardakani Mrs2 , Hassan Bardania Mr2
1- Yasuj University of Medical Sciences , ghasem_ghalamfarsa@yahoo.com
2- Yasuj University of Medical Sciences
Abstract:   (355 Views)
Background & aim: Several natural products have been introduced to protect against a wide range of cancers including colorectal cancer. Dorema aucheri has traditionally been used as an herbal medicine and its extract has been reported to contain significant amounts of antioxidants Such as flavonoids, anthocyanins and phenolic acid. This study aimed to evaluate the cytotoxic and apoptotic effects of Dorema aucheri extract and its liposomal form as a potential cytotoxic agent in SW48 colorectal cancer cell line.
Methods: In this in vitro study, SW48 cell lines were cultured and then, treated with different doses of Hydro-alcoholic extract of Dorema aucheri (DAE), Dorema aucheri-loaded nanoliposomes (Lip-DAE), free liposomes for 24 hours, 48 hours, and 72 hours. The liposomes were prepared using the thin-film dispersion hydration method. Cancer cell survival was assessed using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) at the concentrations of (15/625, 31/25, 62/5, 125, 250 µg/ml). Apoptosis was evaluated by staining cells with Propidium Iodide and Annexin V by flow cytometry. vincristine was used as positive control in this study. Data were analyzed using two-way ANOVA and Tukey post hoc tests.
Results: The result of 50% inhibitory concentration (IC50) in 24 hours was 48.33 µg/ml Lip-DAE, 67.44 µg/ml for DAE and 50 ng/ml for vincristine. DAE and its liposomal form and vincristine reduced the survival of liver cancer cells (SW48) and decreased the cell viability by increasing time and concentration. The results of apoptosis test showed that the highest percentage of apoptosis in this cell line were observed in Lip-DAE than control (41.5%). However, DAE alone was a lower increase than control (38.4%). (p <0.05)
Conclusion: The results of this study showed that loading of DAE in nanoliposomes increases the toxicity and apoptotic effect of DAE and can be a suitable candidate for in-vivo studies to confrontation tumor cells with less side effects.
Keywords: Dorema aucheri, Apoptosis, Nanoliposome, SW48
Type of Study: Research | Subject: Biochemistry
Received: 2020/02/15 | Accepted: 2020/06/30 | Published: 2020/07/7
Send email to the article author

Add your comments about this article
Your username or Email:


XML   Persian Abstract   Print

Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Ghalamfarsa G, rezaeinejad F, Ghasemifard Z, Tajali Ardakani M, bardania H. Hydro-alcoholic extract of Dorema aucheri loaded on liposome nanoparticles, effectively induces apoptosis in SW48 cell line. Armaghane danesh. 2020; 25 (3)
URL: http://armaghanj.yums.ac.ir/article-1-2726-en.html

Volume 25, Issue 3 (6-2020) Back to browse issues page
ارمغان دانش Armaghane danesh
Persian site map - English site map - Created in 0.06 seconds with 32 queries by YEKTAWEB 4162