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:: Volume 25, Issue 1 (2-2021) ::
__Armaghane Danesh__ 2021, 25(1): 0-0 Back to browse issues page
Synthesis Pyrano [2,3-c] Pyrazole-based compounds to induce apoptosis by reducing the expression of anti-apoptotic Bcl-2 protein in human breast cancer MCF-7 cells
Hamideh Shahriyari-Nejad, Negin Pourmohsen, Hossein Ghafouri 1, Sevda Zareie, Roghayeh Sharifi, Nosrat. O Mahmoodi
1- , h.ghafoori@guilan.ac.ir
Abstract:   (838 Views)
Aim: Bcl-2 is a potential target for tumor treatment. The inhibition of the Bcl-2 production is research target of attract in the field of anti-cancer drug development. Recently, the assessment of antitumor activity appeared to be promising for pyrazole derivatives. Therefore, this study was designed to investigate the anti-cancer effects of novel pyrazole derivatives (HN1and HN2.).
Material and methods: The newly synthesized substances were screened in vitro against breast adenocarcinoma (MCF-7). The Western-blot analysis was carried out to study signaling pathways of MCF-7 breast cancer cells. The compounds HN1 and HN2 significantly inhibited the proliferation of human breast cancer cells.
Resault: The compounds HN1and HN2 inhibited the growth of MCF-7 cells with IC50 values of 7.4 μg/ml and 8.68 μg/ml, respectively. In addition, compounds HN1and HN2 (22.5-50 μg/ml) significantly inhibited the anti-apoptotic Bcl-2 protein production. The compound HN2 significantly inhibited Bcl-2 expression in a concentration-dependent manner, corresponding 24% at 37.5 μg/ml, 30% at 50 μg/ml. Also compound HN1 at the same concentrations inhibited Bcl-2 expression by 12%, 0% at 37.5 and 50 μg/ml in MCF-7 cells, respectively.
Conclusion: HN2 could suppress the viability of MCF-7 cells and induce apoptosis in breast cancer cells by down-regulation of anti-apoptotic factor, Bcl-2.
Keywords: pyrazole, apoptosis, MCF-7 cells, Bcl-2
     
Type of Study: Research | Subject: Biochemistry
Received: 2019/08/17 | Accepted: 2019/12/2 | Published: 2020/03/4
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Shahriyari-Nejad H, Pourmohsen N, Ghafouri H, zareie S, Sharifi R, Mahmoodi N O. Synthesis Pyrano [2,3-c] Pyrazole-based compounds to induce apoptosis by reducing the expression of anti-apoptotic Bcl-2 protein in human breast cancer MCF-7 cells. Armaghane danesh. 2021; 25 (1)
URL: http://armaghanj.yums.ac.ir/article-1-2598-en.html


Volume 25, Issue 1 (2-2021) Back to browse issues page
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