[Home ] [Archive]   [ فارسی ]  
:: Main :: About :: Current Issue :: Archive :: Search :: Submit :: Contact ::
:: Volume 23, Issue 5 (11-2018) ::
__Armaghane Danesh__ 2018, 23(5): 577-590 Back to browse issues page
Cytotoxic effects and evaluation of p53gene expression toward gastric cancer AGS cells treated with Palladium nanoparticles
M Mansouri1 , F Baghbani-Arani 2, SA Sadat Shandiz3
1- Department of Genetics and Biotechnology, Varamin-Pishva Branch, Islamic Azad University, Varamin, Iran,
2- Department of Genetics and Biotechnology, Varamin-Pishva Branch, Islamic Azad University, Varamin, Iran, , baghbani.f@gmail.com
3- Department of Biology, Central Tehran Branch, Islamic Azad University, Tehran, Iran.
Abstract:   (393 Views)
Abstract
Background & aim:  Gastric cancer is one of the most common cancers in different parts of the world. Nowadays, nanoparticles, as an anticancer agent, have been considered in research on cancer treatment. Since the P53 gene has been identified as a tumor suppressor gene in many cancers, the aim of this study was to evaluate the expression of P53 gene on AGS gastric cancer cells after the effects of palladium nanoparticles.
 
Methods: The present experimental study was conducted from April to September 2011 in Varamin Islamic Azad University. In this study, AGS and normal HEK293 cancer cells were treated for 48 hours at different concentrations of palladium (5700.570, 57.5, μg / ml) nanoparticles. The effect of nanoparticles on cell survival was measured by MTT method. Extracting RNA and synthesizing of cDNA was determined. Finally, expression of P53 gene was evaluated using Real Time PCR. The collected data were analyzed using one-way ANOVA.
 
Results: The results of cell toxicity indicated that in high concentrations of palladium nanoparticles, cell proliferation significantly lead to cell death  compared to the control group (P = 0.002). The results of the Real Time PCR test disclosed that P53 gene expression was not significantly increased in comparison with the reference gene during 48 hours (P = 0.09).
 
Conclusion: Palladium nanoparticles presented an ability to destroy the AGS cancer cell line compared to the normal HEK293 class, but this was not due to the increased expression of the P53 gene. More studies are desirable to determine the type of cell death caused by the toxicity of palladium nanoparticles.
 
 
Keywords: Palladium nanoparticles, Gastric cancer, P53 gene
Full-Text [PDF 635 kb]   (91 Downloads)    
Type of Study: Research | Subject: Special
Received: 2018/01/6 | Accepted: 2018/11/3 | Published: 2018/11/3
Send email to the article author

Add your comments about this article
Your username or Email:

CAPTCHA code


XML   Persian Abstract   Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Mansouri M, Baghbani-Arani F, Sadat Shandiz S. Cytotoxic effects and evaluation of p53gene expression toward gastric cancer AGS cells treated with Palladium nanoparticles. Armaghane danesh. 2018; 23 (5) :577-590
URL: http://armaghanj.yums.ac.ir/article-1-2058-en.html


Volume 23, Issue 5 (11-2018) Back to browse issues page
ارمغان دانش Armaghane danesh
Persian site map - English site map - Created in 0.06 seconds with 32 queries by YEKTAWEB 3897