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:: Volume 22, Issue 4 (7-2017) ::
__Armaghane Danesh__ 2017, 22(4): 419-430 Back to browse issues page
Study of Nigella sativa seed’s hydroethanolic extract on skin wound healing in male diabetic rats
Elham Norbar 1, Naser Mirazi 2
1- MSc , elhamnorbar909@yahoo.com
2- Phd
Abstract:   (587 Views)

Introduction: Skin wounds are a common event in diabetes. Plants and their extracts known as phytomedicine have immense potential for the management and treatment of  skin wounds. The aim of this study is investigation of effect of Nigella sativa seeds extract (NSE) on skin wound healing in male diabetic rats.
Methods: In this experimental study, 49 Wistar male rats (weighing 220-250 g) were randomly divided into seven groups of equal numbers, including: The control group that were healthy and without medication (A), healthy rats used of phenytoin 1% (B), receiver of eucerine or sham(C), diabetic rats without treatment (D), diabetic rats treated with phenytoin cream 1%(E) and diabetic groups received NSE ointment 20%(F) and 40%(G). Diabete was induced via stereptozotocin 60 mg/kg.  After anesthetization by Ketamine 10% (50mg/kg) + Xylazine 2% (10mg/kg), skin wound (1cm×2cm ) were done on the back of all animals and medical prescription and measuring wounds performed until perfect recovery. For histopathological studies, some biopsies were taken on different days. The data were analyzed using one-way ANOVA and Turkey’s tests.
Results: The average healing time for D and E groups were took place in 27th  and 24th  days after beginning of skin wound respectively. Healing time lasted 23, 24 and 21 days respectively in control and sham and B groups. The shortest time to heal the wound were in diabetic groups received NSE ointment 20 %(18 days) and NSE ointment 40%(15 days).These two groups also had the smallest mean surface of wound. The mean of surface wound in groups that treated with nigella sativa extract showed significant difference with control group (P<0.05).
Conclusion: The results of this study showed that the best healing effect was from nigella sativa extract40% ointment. With regard to anti-inflammation and anti-microbial effects of nigella sativa, it maybe this plant has effects on wound healing process.The antioxidant compounds which present in NSE are able to accelerate the healing process compared to conventional drugs.
Key words: Nigella Sativa, Wound healing, Diabetes, Rat

Keywords: Nigella Sativa, Wound healing, Diabetes, Rat
Full-Text [PDF 915 kb]   (63 Downloads)    
Type of Study: Research | Subject: Physiology
Received: 2017/04/26 | Accepted: 2017/09/21 | Published: 2017/09/28
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norbar E, mirazi N. Study of Nigella sativa seed’s hydroethanolic extract on skin wound healing in male diabetic rats. Armaghane danesh. 2017; 22 (4) :419-430
URL: http://armaghanj.yums.ac.ir/article-1-1779-en.html
Volume 22, Issue 4 (7-2017) Back to browse issues page
ارمغان دانش Armaghane danesh
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