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:: Volume 19, Issue 8 (11-2014) ::
__Armaghane Danesh__ 2014, 19(8): 727-736 Back to browse issues page
Detection of EnteroinvasiveEscherichia coli by PCR technique in Children with Diarrhea
V Aein 1, M Kargar, A Doosti, M Gholami
1- , Vahid_aein@hotmail.com
Abstract:   (2982 Views)
Background & aim: EnteroinvasiveEscherichia coliis one of the important agents of invasion to intestinal epithelial cells, damage and cell death which due to dysentery. The aim of this study wastoDetection of EnteroinvasiveEscherichia coli by PCR technique from Children’s Diarrheain yasuj. Methods:This cross-sectional study was performed on 200 stool samples taken from children with diarrhea in Yasuj. After initial identification of E.coli strains by culture and biochemical tests, EIEC gene such as ipaH detected by PCR technicque and antibiotic susceptibility of isolates was evaluated by using disc diffusion (CLSI) method. Results: Out of all examined samples, 16(8%) EIEC were separated. Antibiotic susceptibility test showed that the most susceptible antibiotic is ciprofloxacin for EIEC and also most resistant antibiotic is ceftizoxime. Conclusion: Results showed that EIEC strains have a moderate prevalence than other studies in our study area. Therefore, for importance of this strain to producing dysentery, hospital-wide surveillance using molecular techniques hase been proposed in other regions of country.
Keywords: EnteroinvasiveE.coli, Enteropathogen, PCR, Antibiotic resistance
Full-Text [PDF 400 kb]   (839 Downloads)    
Type of Study: Research | Subject: Special
Received: 2015/04/4 | Accepted: 2015/04/4 | Published: 2015/04/4
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Aein V, Kargar M, Doosti A, Gholami M. Detection of EnteroinvasiveEscherichia coli by PCR technique in Children with Diarrhea. Armaghane danesh. 2014; 19 (8) :727-736
URL: http://armaghanj.yums.ac.ir/article-1-136-en.html


Volume 19, Issue 8 (11-2014) Back to browse issues page
ارمغان دانش Armaghane danesh
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