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Antibacterial Effects of Ethanolic Extracts of Ziziphus jujuba, Medicago sativa, Reum ribes and Hyssopus officinalis on Some Standard Gram-Positive and Gram-Negative Bacteria in Vitro
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J Sayyahi    1, H Mobayen2 , B Jafari3 , A Jafari-Sales4 |
1- Young Researchers and Elite Club, Ahar Branch, Islamic Azad University, Ahar, Iran , TARANOOM.ISI.ISC@GMAIL.COM
2- Department of Microbiology, Tabriz branch, Islamic Azad University, Tabriz, Iran
3- Department of Microbiology, Ahar Branch, Islamic Azad University, Ahar, Iran
4- Young Researchers and Elite Club, Ahar Branch, Islamic Azad University, Ahar, Iran |
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Abstract: (1963 Views) |
Background & aim: Currently, with increasing resistance to synthetic drugs, the demand for natural and safe alternatives to these types of medications has increased. Therefore, the aim of the present study was to determine and evaluate the antibacterial effects ethanolic extracts of Ziziphus jujuba, Medicago sativa, Reum ribes and Hyssopus officinalis on some standard gram-positive and negative bacteria in vitro.
Methods: The present experimental study was conducted in 2019 in the microbiology laboratory of Islamic Azad University, Ahar Branch, Iran. Plants were identified by botanists after being collected from natural habitats. Extraction was performed by Soxhlet extractor and the antimicrobial effects of the extracts were determined by agar well diffusion and determination of Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) on standard bacteria in vitro. The collected data were analyzed using analysis of variance and Chi-square.
Results: Z. jujuba and M. sativa plant extracts were effective on gram-negative bacteria and extracts of R. ribes and H. officinalis plants were effective on gram-positive bacteria, which with increasing the concentration of extracts. The antibacterial property extracts were dode dependent. MBC / MIC results indicated that Z. jujuba and M. sativa plant extracts were most susceptible to P. aeruginosa and E. coli and R. ribes and H. officinalis extracts were most susceptible to S. aureus and B. cereus.
Conclusion: Z. jujuba, M. sativa, R. ribes and H. officinalis plants had significant antibacterial effects, but to introduce these plants as antibacterial drugs requires more complete and comprehensive in vivo studies.
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| Keywords: Standard Bacteria, Extracts, Antibacterial Effects, Gram-Positive, Gram-Negative |
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Full-Text [PDF 867 kb]
(573 Downloads)
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Type of Study: Research |
Subject:
Microbiology
Received: 2019/08/23 | Accepted: 2021/05/24 | Published: 2021/07/6
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