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:: Volume 20, Issue 9 (12-2015) ::
__Armaghane Danesh__ 2015, 20(9): 768-779 Back to browse issues page
The ultra-structural survey of Aloe Vera extract on photoreceptor layer after methanol intoxication
M Bahramian1 , A Amiri2 , A Zarenezhad1 , M Mard khoshnood1 , E Zarenezhad1 , A Esfandiari 3
1- Noncommunicable Disease Research Center, Fasa University of Medical Sciences, Fasa Iran
2- kazerun Branch, Islamic Azad University, Kazerun, Iran
3- Department of Basic Sciences of Veterinary Medicine, Kazerun Branch, Islamic Azad University, Kazerun, Iran , esfandiari.arash@gmail.com
Abstract:   (5414 Views)

Background & aim: The photoreceptor layer is a main part of the retina. The Aloe Vera has antioxidant, antibacterial and antidiabetic effects. The aim of this study was to investigate the ultra-structure of the photoreceptor layer of male rats under the effect of methanol intoxication and protective effects of Aloe Vera extract against the methanol toxicity. Methods: The present experimental study was conducted on 30 adult male wistar rats in three groups of ten: as control, methanol intoxication, methanol intoxication receiving 400 mg/kg Aloe Vera extract for 30 days. Methanol intoxication was induced by intraperitoneal injection of 4gr/kg of methanol for 30 days. At the end of experiment, the eyes were removed and retina was separated near the optic disc and immersed in gluteraldehyde 4%. The retinal tissue was rinsed with buffer and fixed in osmium tetroxide 1% and dehydrated through a graded alcohol series. Then, the tissues were placed in a mixture of propylene oxide and resin and embedded in pure resin. Semi thin and ultrathin sections prepared and studied by transmission electron and light microscopes. Using One Way ANOVA and Tukey test data were analyzed. Result: Results showed that revealed decrement of the pathological sign in Aloe Vera-methanol group in comparison to methanol intoxication group. The outer segment loss, highly vacuolization in inner segment and condense and pyknotic nuclei were seen in methanol intoxication group. But the moderate outer segment loss, lightly vacuolization in inner segment and condense nuclei were observed in Aloe Vera-methanol group. Morphometric observations showed that the thickness of photoreceptor layer decreased in methanol intoxication group and little reduction in Aloe Vera-methanol group. The decrement of thickness in this group has significant difference with control group. But the decrement of thickness of photoreceptor layer in Aloe Vera-methanol group has no significant difference with control group. Conclusion: These observations showed that methanol caused injury in the photoreceptor layer and Aloe Vera could improve the damage to photoreceptor layer.

Keywords: Photoreceptor layer, Methanol, Aloe Vera
Full-Text [PDF 582 kb]   (1516 Downloads)    
Type of Study: Research | Subject: Anatomy
Received: 2015/08/9 | Accepted: 2015/10/13 | Published: 2015/11/21
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Bahramian M, amiri A, zarenezhad A, mard khoshnood M, zarenezhad E, esfandiari A. The ultra-structural survey of Aloe Vera extract on photoreceptor layer after methanol intoxication. armaghanj 2015; 20 (9) :768-779
URL: http://armaghanj.yums.ac.ir/article-1-1003-en.html


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Volume 20, Issue 9 (12-2015) Back to browse issues page
ارمغان دانش Armaghane Danesh
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