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Effect of Curcumin on Oxidative Stress Status in HepG2 Liver Cells
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Mohadeseh Pouriani   , Fatemeh zahra Ghavamifar , Maryam Salehzadeh , Nasrin Ziamajidi , Roghyeh Abbasalipourkabir , Mahdi Bahmani1 |
| 1- , bahmani.m64@gmail.com |
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Abstract: (80 Views) |
Introduction: In recent years, the utilization of natural compounds has emerged as a prominent research area in cancer prevention. These compounds encompass a wide range of bioactive components, among which the antioxidant potential of curcumin has been reported. Despite the potential benefits of this compound, further investigation into its impact on oxidative stress is warranted. Therefore, this study aimed to evaluate the effects of curcumin on the oxidative stress status in HepG2 human liver cancer cells.
Materials and Methods: Initially, the viability of the HepG2 cell line was assessed after 24 and 48 hours of incubation under the influence of serial curcumin concentrations (1000, 500, 250, 125, 62.5, 31.25, 15.6, 7.8, 3.9, and 0 μM) using the MTT assay. To investigate the oxidative stress status in the aforementioned cell line, the levels of total antioxidant capacity (TAC), total oxidant status (TOS), and malondialdehyde (MDA), as well as the activity of antioxidant enzymes including superoxide dismutase (SOD) and catalase (CAT) were determined using commercially available colorimetric kits.
Results: MTT results indicated that curcumin could significantly reduce the survival of HepG2 cells, with an IC50 of 31.25 μM after 48 hours. Two concentrations, lower and higher than the IC50, were selected for subsequent experiments. Additionally, TOS levels in treated cells decreased (p < 0.01 for 15.6 μM and p < 0.001 for 31.25 and 62.5 μM concentrations) while TAC levels significantly increased (p < 0.05 for 15.6 μM and p < 0.001 for the two higher concentrations). MDA levels also significantly decreased (in the 15.6 μM group with p < 0.01 and in the 31.25 and 62.5 μM groups with p < 0.001). The activity of SOD enzymes (p < 0.01 for 15.6 μM concentration and p > 0.001 for 31.25 and 62.5 μM concentrations) and CAT (p < 0.01 for 15.6 μM concentration and p < 0.001 for the other two groups) also increased in the treated groups compared to the untreated cells.
Conclusion: Based on the obtained results, the antioxidant effects of these compounds were demonstrated by reducing TOS and MDA, and increasing TAC and the activity of antioxidant enzymes in the HepG2 human liver cancer cell line.
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| Keywords: Curcumin, Oxidative Stress, HepG2 Cell Line |
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Type of Study: Research |
Subject:
Biochemistry
Received: 2025/07/7 | Accepted: 2025/10/4 | Published: 2025/12/2
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