|
Investigation of histopathological changes caused by Leishmania major in BALB/c mice treated with aqueous extract of garlic
|
Maryam Rabi moghadam1   , Hossein Rezvan 2, Alireza Nourian1 , Sahar Hamoonnavard1 |
1- Bu-Ali Sina University
2- Bu-Ali Sina University , h.rezvan@basu.ac.ir |
|
|
Abstract: (74 Views) |
Background and purpose
Cutaneous leishmaniasis is a tropical and treatment-resistant disease which the range of treatment strategies based on effective plant substances has been expanded due to the side effects of standard druge. Garlic extract can be investigated as an active phytochemical with therapeutic potential for cutaneous leishmaniasis from various aspects.
Materials and methods
Female BALB/c mice (No: 36) were divided into 6 equal groups, and the standard strain of Leishmania major parasite was injected into 5 groups, three groups were treated with aqueous garlic extract (1.6 g/kg/w) orally and topically, and three groups were treated as control, without treatment and received Glucantim (30mg /d/kg) used as drug. The size of the wound in five groups was evaluated weekly, and after the 90-day treatment, skin tissue around the wound, lymph nodes, spleen, and liver were taken for histopathologic evaluation. A pvalue ≤ 0.05 was considered to be significant for all analyses.
Results
A significant reduction in wound size was observed in the groups receiving the garlic extract orally compared to the control group(p≤0.05), and the structures of the lymphoid organs differed in these two groups in inflammation, granuloma, and lymphocyte populations compared to the control groups and even to the group receiving garlic topical ointment, the severity of inflammation and the presence of granulation tissue, which indicates chronic inflammation, also decreased in the treatment group with oral garlic extract(p≤0.05).
Conclusion
The oral ingestion of aqueous garlic extract can be considered as a natural treatment option in Leishmania infection with its effect on the wound healing process of Leishmaniasis.
|
|
| Keywords: Aqueous extract of garlic, Cutaneous leishmaniasis, Histopathology, BALB/c mice |
|
|
|
Type of Study: Research |
Subject:
Parasitology
Received: 2024/08/17 | Accepted: 2024/11/3 | Published: 2024/11/9
|
|
|
|
|
|
|
| Send email to the article author |
|
|
|
| Add your comments about this article |
|
|
|
