Background and Purpose: Stigma refers to negative and destructive attitudes and beliefs that society has towards some social groups, such as mental patients, disabled people, or a certain race. In Iran, there is no comprehensive and accurate questionnaire about the stigma of physical disability from a sociological point of view. Therefore, the present research was conducted with the aim of designing and measuring physical disability stigma questionnaire. Research Method: The present study is a survey-cross-sectional study that was conducted among 620 people with physical disabilities living in Tehran and Yasouj cities using convenience sampling method. In order to evaluate the validity of the questionnaire, content, face, construct, convergent, discriminant, Cross-validated Communality and redundancy were used, and Cronbach's alpha, loop method, and composite reliability were used to evaluate reliability. Data were analyzed using SPSS and PLS software. Findings: The results showed that convergent validity is established at the level of variables (AVE > 0.50) and at the level of items (factor loadings > 0.40); which showed the validity and accuracy of the investigated items to measure the related variables. According to factor loading values, in measuring social stigma, family stigma dimension (0.770); In the measurement of perceived stigma, the dimension of "feeling seen differently (0.870)" and in the measurement of self-stigma, the dimension of "avoidance stigma (0.871)" played a greater role than other dimensions. It was also shown that divergent validity is established at the level of variables and items, and according to the Fornell-Larcker index, the square root of the extracted average variance of each factor is greater than the maximum correlation of that factor with other factors. The results of combined reliability showed that the questionnaire has acceptable internal consistency reliability and the reliability of the measurement model is confirmed (CR > 0.70). Finally, the results of common cross-validity and redundancy showed that the measurement model has good quality, appropriateness and predictive power (Q2 > 0.11). Conclusion: According to the validity and reliability indicators of the measurement tool, it can be said that this questionnaire is appropriate in terms of content and the items have been able to measure the desired variables. Also, the mentioned questionnaire has a good and favorable quality and can be a proper tool for measuring the stigma of physical disability.