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:: Volume 28, Issue 5 (9-2023) ::
__Armaghane Danesh__ 2023, 28(5): 621-637 Back to browse issues page
The Ability of Pregnenolone in Proliferation of Mouse Neural Stem Cells and Reduction of Inflammatory and Oxidant Markers after Induction of Inflammation with Lipopolysaccharide in Vitro
K Negintaji1 , M Foroozanfar1 , M Jafarinia1 , A Ghanbari 2
1- Department of Biology, Marvdasht Branch, Islamic Azad University, Marvdasht, Iran,
2- Cellular and Molecular Research Center, Yasuj University of Medical Sciences, Yasuj, Iran , amirghanbari52@yahoo.com
Abstract:   (750 Views)
Background & aim: Pregnenolone acts as a precursor to other steroid hormones and exerts its effect as an anti-inflammatory molecule to maintain immune homeostasis in various inflammatory conditions. In these diseases, a decrease in the level ofP has been observed, which emphasizes its role in neuroprotection and nerve regeneration and its anti-inflammatory role. Accordingly, the purpose of the present study was to determine the ability of Pregnenolone in the proliferation of mouse neural stem cells and reduce inflammatory and oxidant markers. of inducing inflammation with lipopolysaccharide in laboratory conditions.

Methods: In the present experimental study conducted at Yasuj University of Medical Sciences, neural stem cells (NSCs) were isolated from the embryonic cortex of E14 mice with standard protocol and incubated for 5 days. Subsequently, neurosphere formation and propagation for second passage the survival of the cells was done after pregnenolone combined treatment with lipopolysaccharide (LPS) inflammatory model. The number of neurospheres and cells derived from neurospheres were counted after 5 days of incubation in the inflammatory model. The supernatant of the cells was removed and the levels of oxidant and antioxidant markers MDA, NO FRAP, and inflammatory markers IL6 and TNFα were measured by ELISA method. Data were analyzed by one-way variance statistical method and Tukey's post hoc test.

Results: The results indicated that pregnenolone with its effect on inflammatory factors could increase the proliferation of neural stem cells in conditions of inflammation and the greatest effect was observed in the group treated with 10 μM dose of pregnenolone with an increase of 68% compared to the LPS group. On the other hand, it caused a decrease in the inflammatory factors TNF-α (12%) and IL-6 (30%) and oxidative stress factors including NO (38%) and MDA (20%) compared to the LPS group, as well as a significant increase FRAP was an antioxidant marker (P<0.0001) in the model of inflammation caused by LPS in the culture medium of mouse neural stem cells.

Conclusion: The results of the present study indicated that Pregnenolone, by affecting inflammatory factors, increased the proliferation of neural stem cells in the conditions of inflammation, and it was as well able to reduce the amount of inflammatory and oxidant markers in the inflammatory model of the culture medium.

 
Keywords: Neural stem cell, Neurosteroid, pregnenolone, Inflammation model, lipopolysaccharide, Oxidative stress
Full-Text [PDF 883 kb]   (153 Downloads)    
Type of Study: Research | Subject: General
Received: 2023/02/22 | Accepted: 2023/09/3 | Published: 2023/09/9
References
1. Lunn JS, Sakowski SA, Hur J, Feldman EL. Stem cell technology for neurodegenerative diseases. Annals of Neurology 2011; 70: 353-61.## [DOI:10.1002/ana.22487] [PMID] []
2. Tian C, Zheng JC. Reprogrammed astrocytes as a potential therapy for neurodegenerative disorders. Science (New York, NY) 2011; 334: 53. ##
3. Saavedra JM. Angiotensin II AT1 receptor blockers as treatments for inflammatory brain disorders.Clinical Science 2012; 123: 567-90. ## [DOI:10.1042/CS20120078] [PMID] []
4. Hosseini M, Zakeri S, Khoshdast S, Yousefian FT, Rastegar M, Vafaee F, et al. The effects of Nigella sativa hydro-alcoholic extract and thymoquinone on lipopolysaccharide-induced depression like behavior in rats. Journal of Pharmacy and Bioallied Sciences 2012; 4: 219. ## [DOI:10.4103/0975-7406.99052] [PMID] []
5. Pourganji M, Hosseini M, Soukhtanloo M, Zabihi H, Hadjzadeh MA. Protective role of endogenous ovarian hormones against learning and memory impairments and brain tissues oxidative damage induced by lipopolysaccharide. Iranian Red Crescent Medical Journal 2014; 16(3):25-8. ## [DOI:10.5812/ircmj.13954] [PMID] []
6. Joshi R, Garabadu D, Teja GR, Krishnamurthy S. Silibinin ameliorates LPS-induced memory deficits in experimental animals, Neurobiology of Learning and Memory 2014; 116: 117-31. ## [DOI:10.1016/j.nlm.2014.09.006] [PMID]
7. González H, Elgueta D, Montoya A, Pacheco R. Neuroimmune regulation of microglial activity involved in neuroinflammation and neurodegenerative diseases. Journal of Neuroimmunology 2014; 274:1-13. ## [DOI:10.1016/j.jneuroim.2014.07.012] [PMID]
8. Nakagawa Y, Chiba K. Role of microglial m1/m2 polarization in relapse and remission of psychiatric disorders and diseases. Pharmaceuticals 2014; 7: 1028-48. ## [DOI:10.3390/ph7121028] [PMID] []
9. Strosznajder RP, Gadamski R, Czapski GA, Jesko H, Strosznajder JB. Poly (ADP-ribose) polymerase during reperfusion after transient forebrain ischemia. Journal of Molecular Neuroscience 2003; 20: 61-71. ## [DOI:10.1385/JMN:20:1:61] [PMID]
10. Emerit J, Edeas M, Bricaire F. Neurodegenerative diseases and oxidative stress. Biomedicine & Pharmacotherapy 2004; 58: 39-46. ## [DOI:10.1016/j.biopha.2003.11.004] [PMID]
11. Stoffel-Wagner B. Neurosteroid metabolism in the human brain. European Journal of Endocrinology 2001; 145: 669-80. ## [DOI:10.1530/eje.0.1450669] [PMID]
12. Chung HT, Pae HO, Choi BM, Billiar TR, Kim YM. Nitric oxide as a bioregulator of apoptosis. Biochemical and Biophysical Research Communications 2001; 282: 1075-79. ## [DOI:10.1006/bbrc.2001.4670] [PMID]
13. Gow AJ, Ischiropoulos H. Nitric oxide chemistry and cellular signaling. Journal of Cellular Physiology 2001; 187: 277-82. ## [DOI:10.1002/jcp.1085] [PMID]
14. Negintaji K, Ghanbari A, Jafarinia M, Zibara K. Pregnenolone enhances the proliferation of mouse neural stem cells and promotes oligodendrogenesis, together with Sox10, and neurogenesis, along with Notch1 and Pax6. Neurochemistry International 2023; 163: 105489. ## [DOI:10.1016/j.neuint.2023.105489] [PMID]
15. Murugan S, Jakka P, Namani S, Mujumdar V, Radhakrishnan G. The neurosteroid pregnenolone promotes degradation of key proteins in the innate immune signaling to suppress inflammation. Journal of Biological Chemistry 2019; 294: 4596-4607. ## [DOI:10.1074/jbc.RA118.005543] [PMID] []
16. Balan I, Beattie MC, O'Buckley TK, Aurelian L, Morrow AL. Endogenous neurosteroid (3α, 5α) 3-hydroxypregnan-20-one inhibits toll-like-4 receptor activation and pro-inflammatory signaling in macrophages and brain. Journal of Scientific Reports 2019; 9: 1-14. ## [DOI:10.1038/s41598-018-37409-6] [PMID] []
17. Lei B, Mace B, Dawson H.N, Warner DS, Laskowitz DT, James ML. Anti-inflammatory effects of progesterone in lipopolysaccharide-stimulated BV-2 microglia. Journal of PloS One 2014; 9: 103969. ## [DOI:10.1371/journal.pone.0103969] [PMID] []
18. Park C, Lee H, Hong S, Molagoda IMN, Jeong JW, Jin CY, et al. Inhibition of lipopolysaccharide-induced inflammatory and oxidative responses by trans-cinnamaldehyde in C2C12 myoblasts. International Journal of Medical Sciences 2021; 18: 2480. ## [DOI:10.7150/ijms.59169] [PMID] []
19. Ghasemi A, Hedayati M, Biabani H. Protein precipitation methods evaluated for determination of serum nitric oxide end products by the Griess assay. Journal of Medical Sciences Research 2007; 2: 29-32. ##
20. Bryan NS, Grisham MB. Methods to detect nitric oxide and its metabolites in biological samples. Free Radical Biology and Medicine 2007; 43: 645-57. ## [DOI:10.1016/j.freeradbiomed.2007.04.026] [PMID] []
21. Zaki SM, Mohamed E, Motawie AG, Fattah SA. N-acetylcysteine versus progesterone on the cisplatin-induced peripheral neurotoxicity. Folia Morphologica 2018; 77: 234-45. ## [DOI:10.5603/FM.a2017.0090] [PMID]
22. Fauziah PN, Maskoen AM, Yuliati T, Widiarsih E. Optimized steps in determination of malondialdehyde (MDA) standards on diagnostic of lipid peroxidation. Padjadjaran Journal of Dentistry 2018; 30: 136-9. ## [DOI:10.24198/pjd.vol30no2.18329]
23. Al-Fawaeir S, Akgul EO, Cayci T, Demirin H, Kurt YG, Aydin I, et al. Comparison of two methods for malondialdehyde measurement. Journal of Clinical and Analytical Medicine 2011; 2: 11-4. ##
24. Benzie F, Strain JJ. The ferric reducing ability of plasma (FRAP) as a measure of "antioxidant power": the FRAP assay, Analytical Biochemistry1996; 239: 70-6. ## [DOI:10.1006/abio.1996.0292] [PMID]
25. Sadeghi A, Bastin AR, Ghahremani H, Doustimotlagh AH. The effects of rosmarinic acid on oxidative stress parameters and inflammatory cytokines in lipopolysaccharide-induced peripheral blood mononuclear cells. Molecular Biology Reports 2020; 47: 3557- 66. ## [DOI:10.1007/s11033-020-05447-x] [PMID]
26. Yang JW, Mao B, Tao RJ, Fan LC, Lu HW, Ge BX, Xu JF. Corticosteroids alleviate lipopolysaccharide‐induced inflammation and lung injury via inhibiting NLRP3‐inflammasome activation, Journal of Cellular and Molecular Medicine 2020; 24: 12716-725. ## [DOI:10.1111/jcmm.15849] [PMID] []
27. Veiga S, Garcia‐Segura LM, Azcoitia I. Neuroprotection by the steroids pregnenolone and dehydroepiandrosterone is mediated by the enzyme aromatase. Journal of Neurobiology 2003; 56: 398-406. ## [DOI:10.1002/neu.10249] [PMID]
28. Borowicz K, Czuczwar S, Piskorska B, Banach M. Neuroprotective actions of neurosteroids. Frontiers in Endocrinology 2011; 2: 38- 50. ## [DOI:10.3389/fendo.2011.00050] [PMID] []
29. Fontaine-Lenoir V, Chambraud B, Fellous A, David S, Duchossoy Y, Baulieu EE, Robel P, Microtubule-associated protein 2 (MAP2) is a neurosteroid receptor. Proceedings of the National Academy of Sciences 2006; 103: 4711- 6. ## [DOI:10.1073/pnas.0600113103] [PMID] []
30. [30.Mayo W, Lemaire V, Malaterre J, Rodriguez J, Cayre M, Stewart M, et al. Pregnenolone sulfate enhances neurogenesis and PSA-NCAM in young and aged hippocampus, Neurobiology of Aging 2005; 26: 103-14. ## [DOI:10.1016/j.neurobiolaging.2004.03.013] [PMID]
31. Xu B, Yang R, Chang F, Chen L, Xie G, Sokabe M, Chen L. Neurosteroid PREGS protects neurite growth and survival of newborn neurons in the hippocampal dentate gyrus of APPswe/PS1dE9 mice. Current Alzheimer Research 2012; 9: 361-72. ## [DOI:10.2174/156720512800107591] [PMID]
32. Badran SA, Fayyaz S, Muhammad BT, Choudhary MI. Effect of Steroidal Hormone Pregnenolone on Proliferation and Differentiation of MC3T3-E1 osteoblast like cells. Letters in Drug Design & Discovery 2020; 17: 1139-45. ## [DOI:10.2174/1570180817666200204110859]
33. Grigoryev DN, Long BJ, Njar VC, Brodie AH. Pregnenolone stimulates LNCaP prostate cancer cell growth via the mutated androgen receptor. The Journal of steroid biochemistry and Molecular Biology 2000; 10: 56-75. ## [DOI:10.1016/S0960-0760(00)00131-X] [PMID]
34. Birben E, Sahiner U.M, Sackesen C, Erzurum S, Kalayci O. Oxidative stress and antioxidant defense. World Allergy Organization Journal 2012; 5: 9-19. ## [DOI:10.1097/WOX.0b013e3182439613] [PMID] []
35. Mirzaei F, Khazaei M. Role of nitric oxide in biological systems: a systematic review. Journal of Mazandaran University of Medical Sciences 2017; 27: 192-222. ##
36. Kummer MP, Hermes M, Delekarte A, Hammerschmidt T, Kumar S, Terwel D, et al. Nitration of tyrosine 10 critically enhances amyloid β aggregation and plaque formation. Neuron 2011; 71: 833-44. ## [DOI:10.1016/j.neuron.2011.07.001] [PMID]
37. [37] Naylor J.C, Kilts J.D, Hulette C.M, Steffens D.C, Blazer D.G, Ervin, Strauss J.L, Allen T.B, Massing M.W, Payne V.M, Allopregnanolone levels are reduced in temporal cortex in patients with Alzheimer's disease compared to cognitively intact control subjects, Biochimica et Biophysica Acta (BBA)-Molecular and Cell Biology of Lipids 2010; 1801 :951-59. ## [DOI:10.1016/j.bbalip.2010.05.006] [PMID] []
38. Vallée M. Neurosteroids and potential therapeutics: focus on pregnenolone, The Journal of Steroid Biochemistry and Molecular Biology 2016; 160: 78-87. ## [DOI:10.1016/j.jsbmb.2015.09.030] [PMID]
39. [39.de Pinto MC, Tommasi F, De Gara L. Changes in the antioxidant systems as part of the signaling pathway responsible for the programmed cell death activated by nitric oxide and reactive oxygen species in tobacco Bright-Yellow 2 cells. Plant Physiology 2002; 130: 698-708. ## [DOI:10.1104/pp.005629] [PMID] []
40. Andrabi SS, Kaushik P, Mumtaz SM, Alam MM, Tabassum H, Parvez S. Pregnenolone attenuates the ischemia-induced neurological deficit in the transient middle cerebral artery occlusion model of rats., ACS Omega 2022; 7: 19122-130. ## [DOI:10.1021/acsomega.1c07016] [PMID] []
41. Baptista P, Andrade JP. Adult hippocampal neurogenesis: regulation and possible functional and clinical correlates. Frontiers in Neuroanatomy 2018; 12: 44. ## [DOI:10.3389/fnana.2018.00044] [PMID] []
42. Daugherty DJ, Selvaraj V, Chechneva OV, Liu XB, Pleasure DE, Deng W. A TSPO ligand is protective in a mouse model of multiple sclerosis. EMBO Molecular Medicine 2013; 5: 891-903. ## [DOI:10.1002/emmm.201202124] [PMID] []
43. Morsy MA, Abdel-Gaber SA, Mokhemer SA, Kandeel M, Sedik WF, Nair AB, et al. Pregnenolone Inhibits doxorubicin-induced cardiac oxidative stress, inflammation, and apoptosis-role of matrix metalloproteinase 2 and nadph oxidase 1. Pharmaceuticals 2023; 16: 665. ## [DOI:10.3390/ph16050665] [PMID] []
44. Higuchi M, Ji B, Maeda J, Sahara N, Suhara T. In vivo imaging of neuroinflammation in Alzheimer's disease. Clinical and Experimental Neuroimmunology 2016; 7: 139-44. ## [DOI:10.1111/cen3.12308]
45. Reed-Geaghan EG, Savage JC, Hise AG, Landreth GE. CD14 and toll-like receptors 2 and 4 are required for fibrillar Aβ-stimulated microglial activation. Journal of Neuroscience 2009; 29: 11982-92. ## [DOI:10.1523/JNEUROSCI.3158-09.2009] [PMID] []
46. Rhen T, Cidlowski JA. Antiinflammatory action of glucocorticoids-new mechanisms for old drugs. New England Journal of Medicine 2005; 353: 1711-23. ## [DOI:10.1056/NEJMra050541] [PMID]
47. Mansell A, Smith R, Doyle SL, Gray P, Fenner JE, Crack PJ, et al. Suppressor of cytokine signaling 1 negatively regulates Toll-like receptor signaling by mediating Mal degradation, Nature Immunology 2006; 7: 148-55. ## [DOI:10.1038/ni1299] [PMID]
48. Weng JH, Liang MR, Chen CH, Tong SK, Huang TC, Lee SP, et al. Pregnenolone activates CLIP-170 to promote microtubule growth and cell migration. Nature Chemical Biology 2013; 9: 636-42. ## [DOI:10.1038/nchembio.1321] [PMID]
49. Brinton RD, Thompson RF, Foy MR, Baudry M, Wang J, Finch CE, et al. Progesterone receptors: form and function in brain. Frontiers in Neuroendocrinology 2008; 29: 313-39. ## [DOI:10.1016/j.yfrne.2008.02.001] [PMID] []
50. Ferri C, Fioranelli M. The role of pregnenolone in inflammatory degenerative brain disease. Interdiscip J Microinflammation 2014, 1: 2. ##
51. Larti F, Kahrizi K, Musante L, Hu H, Papari E, Fattahi Z, et al. Erratum: A defect in the CLIP1 gene (CLIP-170) can cause autosomal recessive intellectual disability. European Journal of Human Genetics 2015; 23 :416-16. ## [DOI:10.1038/ejhg.2014.152] [PMID] []
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Negintaji K, Foroozanfar M, Jafarinia M, Ghanbari A. The Ability of Pregnenolone in Proliferation of Mouse Neural Stem Cells and Reduction of Inflammatory and Oxidant Markers after Induction of Inflammation with Lipopolysaccharide in Vitro. armaghanj 2023; 28 (5) :621-637
URL: http://armaghanj.yums.ac.ir/article-1-3448-en.html


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Volume 28, Issue 5 (9-2023) Back to browse issues page
ارمغان دانش Armaghane Danesh
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