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:: Volume 27, Issue 5 (8-2022) ::
__Armaghane Danesh__ 2022, 27(5): 0-0 Back to browse issues page
Evaluation of abundance of broad spectrum beta-lactamase in urinary isolates of Klebsiella pneumoniae in Isfahan Educational hospital by phenotypic and genotypic methods
Fereshte Ghandehari 1, Mahsa Harati2 , Mojgan Ghiasian2
1- 1Department of Microbiology, Falavarjan Branch, Islamic Azad University, Isfahan, Iran , Fe_gh_2010@yahoo.com
2- 1Department of Microbiology, Falavarjan Branch, Islamic Azad University, Isfahan, Iran
Abstract:   (39 Views)
Abstract: There are several reports regarding the increasing and increasing prevalence of ESBLs-producing organisms from different regions of the country, so that the increasing prevalence of these organisms is a clinical threat and has caused concern among physicians in the treatment of infections caused by these bacteria. . Due to the fact that Klebsiella pneumoniae accounts for a significant percentage of nosocomial and out-of-hospital infections, therefore, understanding the pattern of resistance and susceptibility of this bacterium to beta-lactam antibiotics is important in the treatment and control of infections caused by this bacterium. Methods: In this study, 100 strains of Klebsiella pneumoniae were isolated from urine samples of patients with urinary tract infections from Al-Zahra Hospital in Isfahan. Identification and confirmation of Klebsiella pneumoniae strains were performed using standard biochemical tests. The pattern of antibiotic susceptibility was assessed by disk diffusion method based on CLSI. PCR test was performed with specific primers to identify blaSHV and bla TEM. Results: Out of 100 isolates 33 isolates showed resistance to at least 4 antibiotics with the highest resistance to cefotaxime (38%) and the lowest antibiotic resistance to cotrimaxazole (22%). The combination showed that 10 isolates (86.8%) are broad-spectrum β-lactamase generators. Of these, 7 isolates (70.0%) had SHV gene and the other 3 isolates did not have any of the two TEM and SHV genes. Conclusion: The results indicate a higher frequency of ESBLs and also a higher prevalence of antibiotic resistance in Klebsiella pneumoniae bacteria isolated from urinary tract infections.
Keywords: Klebsiella pneumoniae, Urinary tract infection, Broad-spectrum beta-lactamases, phenotypic and genotypic methods
     
Type of Study: Research | Subject: Microbiology
Received: 2022/05/11 | Accepted: 2022/09/12 | Published: 2022/09/12
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ghandehari F, harati M, ghiasian M. Evaluation of abundance of broad spectrum beta-lactamase in urinary isolates of Klebsiella pneumoniae in Isfahan Educational hospital by phenotypic and genotypic methods. ارمغان دانش. 2022; 27 (5)
URL: http://armaghanj.yums.ac.ir/article-1-3305-en.html


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Volume 27, Issue 5 (8-2022) Back to browse issues page
ارمغان دانش Armaghane Danesh
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