[Home ] [Archive]   [ فارسی ]  
:: Main :: About :: Current Issue :: Archive :: Search :: Submit :: Contact ::
Main Menu
Home::
Journal Information::
Articles archive::
For Authors::
For Reviewers::
Registration::
Contact us::
Site Facilities::
Indexing & Abstracting::
Publication Ethics::
::
Search in website

Advanced Search
..
Receive site information
Enter your Email in the following box to receive the site news and information.
..
Indexing & Abstracting
DOAJ
GOOGLE SCHOLAR
..
:: Volume 27, Issue 5 (8-2022) ::
__Armaghane Danesh__ 2022, 27(5): 578-589 Back to browse issues page
Evaluation of the Frequency of Broad-Spectrum Beta-Lactamases in Urinary Isolates of Drug-Resistant Klebsiella pneumoniae by Genotypic and Phenotypic Methods
M Herati1 , F Ghandahari 2, M Qalazian1
1- Department of Microbiology, Flaverjan Branch, Islamic Azad University, Isfahan, Iran
2- Department of Microbiology, Flaverjan Branch, Islamic Azad University, Isfahan, Iran , Fe_gh_2010@yahoo.com
Abstract:   (773 Views)
Background & aim:  There are several reports regarding the increase and growing prevalence of ESBLs producing organisms from different regions in the country. Considering that Klebsiella pneumoniae includes a significant percentage of hospital infections, therefore, knowing the pattern of resistance and sensitivity of this bacterium to beta-lactam antibiotics plays a significant role in the treatment and control of infections caused by this bacterium. As a result, the aim of the present study was to determine and evaluate the frequency of beta-lactamases The broad spectrum of Klebsiella pneumoniae urinary isolates was drug-resistant by genotypic and phenotypic methods.

Methods: In the present cross-sectional descriptive study conducted in 2018, 100 strains of Klebsiella pneumoniae were isolated from urine samples of patients with urinary tract infection from Al-Zahra Hospital, Isfahan. Klebsiella pneumoniae strains were identified and confirmed using standard biochemical tests. Antibiotic sensitivity pattern was investigated by disk diffusion method based on CLSI criteria. To perform the phenotypic verification test of broad-spectrum β-lactamase producing strains, the combined disc method was used based on CLSI criteria. PCR test was done with specific primers to identify blaSHV and bla TEM. The collected data were analyzed using one-way and independent one-way analysis of variance statistical tests.

Results: Among 100 isolates related to urinary tract infection, 33 isolates indicated resistance to at least 4 antibiotics, the highest resistance to cefotaxime antibiotic (38%) and the least antibiotic resistance to cotrimaxazole (22%). The results of combined disk indicated that 10 isolates (86.8%) were broad-spectrum β-lactamase producers. Among them, 7 isolates (70%) had the SHV gene and the other 3 isolates did not have any of the TEM and SHV genes.

Conclusion: The results indicated a higher frequency of ESBLs and correspondingly a higher prevalence of antibiotic resistance in Klebsiella pneumoniae bacteria isolated from urinary tract infections.

 
Keywords:  Klebsiella pneumoniae, urinary infection, broad-spectrum beta-lactamases, genotypic and phenotypic methods
Full-Text [PDF 829 kb]   (205 Downloads)    
Type of Study: Research | Subject: Microbiology
Received: 2022/05/11 | Accepted: 2022/09/12 | Published: 2022/09/12
Send email to the article author

Add your comments about this article
Your username or Email:

CAPTCHA



XML   Persian Abstract   Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Herati M, Ghandahari F, Qalazian M. Evaluation of the Frequency of Broad-Spectrum Beta-Lactamases in Urinary Isolates of Drug-Resistant Klebsiella pneumoniae by Genotypic and Phenotypic Methods. armaghanj 2022; 27 (5) :578-589
URL: http://armaghanj.yums.ac.ir/article-1-3305-en.html


Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Volume 27, Issue 5 (8-2022) Back to browse issues page
ارمغان دانش Armaghane Danesh
Persian site map - English site map - Created in 0.05 seconds with 39 queries by YEKTAWEB 4645