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:: Volume 26, Issue 4 (9-2021) ::
__Armaghane Danesh__ 2021, 26(4): 0-0 Back to browse issues page
Molecular identification and phylogenetic analysis of Fleas Ctenocephalides canis and Pulex irritans From West and Northwest of Iran based on cytochrome oxidase I
Moussa Tavassoli 1, Shahin Seidi , Farnaz Malelifard
1- , m.tavassoli@urmia.ac.ir
Abstract:   (66 Views)
Background: Fleas of Pulicidae family are the most common ectoparasites infesting humans and domestic livestock throughout the world. addition to eating blood, these insects are carriers of some pathogens such as Yersinia pestis, Rickettsia typhi, and Bartonella hensle for humans and animals. The aim of this study was to investigate the fauna of fleas and show the molecular differences between fleas of the genera Ctenocephalides canis and Pulex irritans based on the mitochondrial marker of cytochrome oxidase I in West and Northwest of Iran.
Materials and methods: The present study is a descriptive cross-sectional and phonetic study that, considering the prevalence of 10% and 95% confidence level and 5% error rate, for 13 months from May 2018 to June 2019 in five provinces, Kermanshah, Kurdistan, Azerbaijan Western, Lorestan and Hamedan. In this study, samples were collected by optical trap, human prey and direct isolation of the sample from the host. The samples were kept in 70% alcohol and identified in the parasitology laboratory using valid diagnostic keys. After DNA extraction and a PCR reaction, 20 samples were sent to Takapouzist Company for sequencing.
Results: The collected samples included 918(47/39%) fleas of C.canis and 1019 (52/60%)fleas of P.irritant. The results of morphological studies showed that there is no intraspecific difference between two species of fleas isolated from different hosts. However, the intraspecific difference based on the molecular marker of cytochrome oxidase I for ten studied populations was 0.15% in P.irritans and zero in C.canis. The difference between two genus of C. canis and P. irritans based on the molecular marker of cytochrome oxidase I was 14%.
Conclusion: No significant difference was observed in morphological characteristics of samples collected from different hosts. However, there were slight differences based on mitochondrial markers in the study populations. The results from the phylogenetic tree based on mitochondrial markers showed that despite the slight differences in this sequence of different hosts and cities, all samples from different regions are in the same phylogeny. The results of mitochondrial genome analysis showed that these pieces are useful for demonstrating intraspecific similarity, and differentiation at species level and genus.
Keywords: Ctenocephalides canis, Flea, Iran, ITS1, ITS2, COX1
     
Type of Study: Research | Subject: Parasitology
Received: 2021/05/16 | Accepted: 2021/08/23 | Published: 2021/09/6
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tavassoli M, seidi S, malelifard F. Molecular identification and phylogenetic analysis of Fleas Ctenocephalides canis and Pulex irritans From West and Northwest of Iran based on cytochrome oxidase I. Armaghane danesh. 2021; 26 (4)
URL: http://armaghanj.yums.ac.ir/article-1-3085-en.html


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Volume 26, Issue 4 (9-2021) Back to browse issues page
ارمغان دانش Armaghane danesh
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