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The Effect of Estradiol on Oxidative Stress Index and Nitric Oxide Levels in Unilateral Ureteral Obstruction in Ovariectomized Rats
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Z Lak    1, M Nematbakhsh2 , A Vahdati3 |
1- Department of Biology, Shiraz Branch, Islamic Azad University, Shiraz, Iran , ZAHRALAK66@YAHOO.COM
2- Water and Electrolyte Research Center, Isfahan University of Medical Sciences, Isfahan, Iran
3- Department of Biology, Shiraz Branch, Islamic Azad University, Shiraz, Iran |
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Abstract: (949 Views) |
Background & aim: Unilateral ureteral obstruction (UUO) is a common disorder of the renal system that may cause irreversible kidney damage and depends on the duration of the obstruction and the extent of renal tissue damage and dysfunction. The aim of the present study was to determine the effect of estradiol on oxidative stress index and nitric oxide levels in unilateral ureteral obstruction in ovariectomized rats.
Methods: In the present experimental study conducted in 2017, 84 rats underwent ovariectomy (180 20 20 g) and were randomly divided into 14 groups of 6. Group 1 (sham group) had no surgery and all groups 2 to 14 had unilateral ureteral obstruction and some of them were treated with medication. Thus; Groups 2-4 were sacrificed 3 days after UUO and receiving the drug (sesame oil, 0.1 and 0.5 mg / kg estradiol), respectively, group 5-7 3 days after UUO and receiving the unblocked drug (RUUO) were sacrificed the next day, groups 8-9 and 10-12 were sacrificed 1 and 3 days after RUUO and estradiol, respectively, and groups 14-13 were sacrificed 3 days after UUO and RUUO and received the drug in both models. Were. Quantitative detection tests for stable metabolites of nitric oxide and malondialdehyde in blood and tissue samples were performed using special kits. The collected data were analyzed using statistical tests and LSD test.
Results: The results of the present study indicated that in the UUO model, the level of nitrite and MDA of renal tissue in the obstructive groups decreased significantly compared to the sham group (p <0.05). However, administration of different doses of estradiol did not alter serum and tissue levels of nitrite and MDA between the treatment and control groups in the UUO and RUUO models (p> 0.05).
Conclusion: According to the obtained results, it can be stated that estradiol administration during UUO and RUUO times had no effect on nitrite and MDA levels. Although its protective effect may occur after a longer period of time than RUUO, the short-term damage caused by UUO can develop even after RUUO.
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| Keywords: Estradiol, Unilateral ureteral obstruction, Nitric oxide, Malondialdehyde |
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Full-Text [PDF 1509 kb]
(296 Downloads)
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Type of Study: Research |
Subject:
Physiology
Received: 2020/10/25 | Accepted: 2021/08/23 | Published: 2021/09/6
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