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Prevalence of Human Papillomavirus in Women with Abnormal Pap smear Referred to Shahid Mofatteh Clinic by Multiplex PCR & Reverse Dot Blot Hybridization in Yasuj, Iran
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P Ghaffari    1, R Jansar2 , M Zol-Adl3 , R Ghadami4 |
1- Department of Obstetrics and Gynecology, Yasuj University of Medical Sciences, Yasuj, Iran , pari.ghaffari@gmail.com
2- Departments of Pathology, Yasuj University of Medical Sciences, Yasuj, Iran
3- Departments of Psychiatry, Yasuj University of Medical Sciences, Yasuj, Iran
4- Student Research Committees, Yasuj University of Medical Sciences, Yasuj, Iran |
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Abstract: (1551 Views) |
Background & aim: Human papillomavirus is the most common sexually transmitted disease and the main risk factor for malignant and malignant lesions in the cervix. The aim of the present study was to determine and evaluate the prevalence of human papillomavirus in women with abnormal pap smear referred to Shahid Mofatteh specialized clinic by Multiplex PCR & Reverse Dot Blot Hybridization in Yasuj, Iran.
Methods: The present descriptive-analytical study was conducted during the years 2015-2016. A total of 67 abnormal cervical cytology specimens including 51 ASC-US specimens, 9 LSIL specimens and 7 HSIL specimens were collected and analyzed by Multiplex PCR & Reverse Dot Blot Hybridization. The collected data were analyzed using chi-square test
Result: The overall prevalence of human papillomavirus (HPV) was 61.2%. 36 (87.8%) women were infected with high-risk types of human papillomavirus, 26 (63.4%) with low-risk types and 2 (4.9%) with unknown types. The most common high-risk types were HPV 39 (43.9%), HPV66 (31.7%), HPV31 (26.8%) and HPV18 (14.6%), respectively. HPV16 was detected in only 2 samples (4.9%).
Conclusion: The results of the present study indicated a high prevalence of papillomavirus infection in women with abnormal Pap smear. In addition, different high-risk genotypes of this virus were identified compared to other studies in Iran. Further samples from wider geographical areas are needed to study the molecular epidemiology and genotypes of human papillomavirus.
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| Keywords: Human Papillomavirus, High Risk Type, Abnormal Pap smear, PCR |
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Full-Text [PDF 978 kb]
(537 Downloads)
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Type of Study: Research |
Subject:
Gynaecology
Received: 2020/09/26 | Accepted: 2021/05/9 | Published: 2021/07/6
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