[Home ] [Archive]   [ فارسی ]  
:: Main :: About :: Current Issue :: Archive :: Search :: Submit :: Contact ::
Main Menu
Home::
Journal Information::
Articles archive::
For Authors::
For Reviewers::
Registration::
Contact us::
Site Facilities::
Indexing & Abstracting::
Publication Ethics::
::
Search in website

Advanced Search
..
Receive site information
Enter your Email in the following box to receive the site news and information.
..
Indexing & Abstracting
DOAJ
GOOGLE SCHOLAR
..
:: Volume 23, Issue 6 (1-2019) ::
__Armaghane Danesh__ 2019, 23(6): 737-746 Back to browse issues page
Identification of Blastocystis sp. subtypes from human using 18s rRNA in Northwest of Iran
J Mohamadi1 , J Hallaj Zadeh2 , M Rostami3 , S Raeghi4 , H Mirahmadi5 , F Bahrami6 , R Shafiei 7, A Bozorgomid8
1- Department of Health affairs, Shiraz University of Medical Sciences, Shiraz, Iran
2- Department of Laboratory Sciences, Maragheh University of Medical Sciences, Maragheh, Iran,
3- Student Research Committee, Maragheh University of Medical Sciences, Maragheh, Iran
4- Department of Laboratory Sciences, Maragheh University of Medical Sciences, Maragheh, Iran
5- Infectious Tropical Medicine Research Diseases and Center, Zahedan University of Medical Sciences, Zahedan, Iran,
6- Zoonoses research center, Kurdistan University of Medical Sciences, Sanandaj, Iran
7- Department of Pathobiology, School of Medicine, North Khorasan University of Medical Sciences, Bojnurd, Iran, , reza_shafi@yahoo.com
8- Department of Parasitology, Asadabad School of Medical Sciences, Asadabad, Iran.
Abstract:   (4268 Views)
Abstract
 
Background & aim: Blastocystis sp. is an anaerobic parasite which lives in the digestive system and can be a zoonotic parasite in different hosts. This study was carried out to identify subtypes and to investigate the genetic variation of Blastocystis sp. from human samples in Urmia, Tabriz & Maragheh cities Northwest of Iran.
 
Methods: In the present descriptive-cross sectional study, 300 human stools from January to October 2017 were randomly selected from treatment centers in North West of Iran. Based on microscopic observation, positive samples examined by DNA barcoding methods for detection of Blastocystis sp. subtypes and their sequences were analyzed. 
 
Results: of the 300 samples, 22 specimens diagnosed as Blastocystis sp. with the microscopic method. Sixteen apart isolates from the positive cases obtained by the molecular method (PCR) and the sequences of the products were examined. Three types of subtypes including ST1, ST2 and ST3 obtained from these samples. One of the samples after two different sequencings, was reported as both of the ST1 and ST3 subspecies, but re-sequencing of the dominant show ST3.
 
Conclusion: There are various subtypes of this parasite in this area. Due to the nature of the subtypes, the zoonotic cycle of this parasite exists in this region. By identifying and determining the Blastocysts subspecies in different hosts as a zoonotic organism, it is possible to detect genetic migration and parasite transmission. It seems that the parasite subtype pattern can be considered in future studies in relation to clinical manifestations.
  
Keywords: Blastocystis sp., subtypes, 18s rRNA, Northwest, Iran
Full-Text [PDF 286 kb]   (960 Downloads)    
Type of Study: Research | Subject: Parasitology
Received: 2018/06/25 | Accepted: 2018/11/3 | Published: 2018/12/25
Send email to the article author

Add your comments about this article
Your username or Email:

CAPTCHA


XML   Persian Abstract   Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Mohamadi J, Hallaj Zadeh J, Rostami M, Raeghi S, Mirahmadi H, Bahrami F, et al . Identification of Blastocystis sp. subtypes from human using 18s rRNA in Northwest of Iran. armaghanj 2019; 23 (6) :737-746
URL: http://armaghanj.yums.ac.ir/article-1-2232-en.html


Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Volume 23, Issue 6 (1-2019) Back to browse issues page
ارمغان دانش Armaghane Danesh
Persian site map - English site map - Created in 0.06 seconds with 39 queries by YEKTAWEB 4645