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:: Volume 21, Issue 5 (8-2016) ::
__Armaghane Danesh__ 2016, 21(5): 435-445 Back to browse issues page
The Effect of Propiconazole and Protective Effects of Selenium Gene Expression Profile of Caspase 9 in the Testicular Tissue of Male Sprague Dawley (SD) Rats
S Rashidi pouya 1, H Mohseni kouchesfehani 2, SA Angaji 3
1- Department of Animal Biology, Faculty of Biological Sciences, Kharazmi University, Tehran, Iran
2- Department of Animal Biology, Faculty of Biological Sciences, Kharazmi University, Tehran, Iran , kouchesfehani@yahoo.com
3- Department of Cell & Molecular Biology, Faculty of Biological Sciences, Kharazmi University, Tehran, Iran
Abstract:   (1704 Views)

Background & aim: Conazoles including imidazoles or triazoles are anti- fungal agents widely used to prevent fungal growth and their infections. Propiconazole placed in this group is a systemic fungicide used widely for detoxification of cereal seeds especially rice in Iran and other countries. This fungicide were designed to inhibit a specific cytochrome P450, CYP51 (lanosterol-14-α- demethylase), a critical step in the biosynthesis of ergosterol, a steroid required for the formation of the fungal cell wall. In the present experimental study, the effect of propiconazole on Caspase 9 gene expression profile as an initiator of apoptotic process and protective effect of selenium were investigated.

Methods: Forty SD rats were divided into 10 groups of 4,  including : control , sham1 (solvent of propiconazole, distilled water), sham 2 (solvent of selenium, normal saline) and 1 group received 0.5 mg/kg selenium ,3 groups received propiconazole in doses of 10,50,75  mg/kg and 3 groups received propiconazole in doses of 10,50,75 mg/kg propiconazole with 0.5 mg/kg of selenium. Injections were intrapritoneal for two weeks in alternate days. Then, using RT-PCR and Total Lab program gene expression of caspase-9 testicular of all groups were studied. Data were analyzed using descriptive statistics.

Results:  A significant increase of caspase 9 expression were observed among all experimental groups compared to control and sham groups. These findings indicated that 0.5 mg/kg selenium is not a suitable dose to create protection in this experimental study. 

Conclusion: The significant increase in Caspase 9 gene expression profile observed in all experimental groups as compared to control suggests activation of apoptosis and inefficacy of selenium to protect the testis against induced damages.

Keywords: Apoptosis, CASP 9, Propiconazole, SD rat, Selenium
Full-Text [PDF 506 kb]   (677 Downloads)    
Type of Study: Research | Subject: Special
Received: 2016/04/17 | Accepted: 2016/07/7 | Published: 2016/07/30
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Rashidi pouya S, Mohseni kouchesfehani H, Angaji S. The Effect of Propiconazole and Protective Effects of Selenium Gene Expression Profile of Caspase 9 in the Testicular Tissue of Male Sprague Dawley (SD) Rats. Armaghane danesh. 2016; 21 (5) :435-445
URL: http://armaghanj.yums.ac.ir/article-1-1316-en.html


Volume 21, Issue 5 (8-2016) Back to browse issues page
ارمغان دانش Armaghane danesh
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