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Comparison of Two Methods of Fixation with Carnoy`s Solution and 96% Ethyl Alcohol in Pap Smear Slides
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KH Abdali   1, M Shamsi , N Riyaz Montazer , HR Tabatabaee |
| 1- , abdali_kh@yahoo.com |
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Abstract: (5121 Views) |
| ABSTRACT:
Introduction & Objective: Cervical carcinoma is a significant health care problem world wide. The Papanicolaou test is the most common effective tool available for early detection of cervical cancer. Blood could have a negative influence on the quality of the pap smear. Good fixation reduces red blood cells of the back ground slides and can help to improve this technique. One of the effective solutions for hemolyzing red blood cells is using Carnoys solution. The aim of this study was to compare two methods of fixation with Carnoys solution and 96% ethyl alcohol in bloody pap smear slides.
Material & Methods: This study was done on 450 bloody slides of pap smear prepared from cervical cells of 225 women who referred for annual screening of cervical cancer from 1384 -85 in Zeinabie and Faghihi hospitals in Shiraz. In each case, after observation of contact bleeding, two samples with conventional pap smear method were prepared. In the first method one of the samples was fixed in 96% ethyl alcohol for 5 minute and another sample was fixed with Carnoys solution for 20 minutes. Then the slides were stained with papanicolaou method and were evaluated by two pathologists. Collected data were analyzed with Fishers exact test, Pearson chi – square test and Independent sample T test.
Results: Results of this study showed that presence of squamous cells and glandular cells in Carnoys fixed slides was more than alcohol – fixed slides. The reduction of red blood cells of the back ground of slides and increase of slide clearance in Carnoys fixed slides were more than alcohol fixed slides. The diagnosis of inflammatory cells and pathogen microorganisms in Carnoys fixed slides were more than alcohol fixed slides but diagnosis of epithelial cells and glandular cells abnormalities in two methods was not statistically significant.
Conclusion: Carnoys stain can be effectively used for proper fixation of Pap smear slides. |
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| Keywords: KEYWORDS: Fixation, Carnoy`s, 96% Ethyl alcohol, Bloody Pap Smear |
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Full-Text [PDF 952 kb]
(1440 Downloads)
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Type of Study: Research |
Subject:
Special
Received: 2015/06/2 | Accepted: 2015/06/2 | Published: 2015/06/2
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