Abstract
Background and aim: Extreme endurance exercises lead to oxidative stress in athletes. Therefore, the purpose of this study was to investigate the effect of curcumin supplement supplementation and light resistance training on the activity of SOD and MDA levels of male Wistar rats during a 8-week endurance training.

Methods: In the present experimental study, 36 male Wistar rats were randomly assigned into one of six control groups, curcumin, endurance training, exercise, after one week of information (age 9 weeks and weight 255.62 ± 19.69 grams). Endurance + resistance, endurance training + curcumin and endurance training + curcumin + resistance. Incremental endurance training (8 weeks, 5 sessions per week) was performed on a special treadmill. Speed ​​and running time in the last week reached 35 m / min and 70 minutes. Resistance training (8 weeks, 2 sessions per week) was performed on vertical ladder by closing the rat's weight to the tail. Rats received supplemental curcumin by intraperitoneal injection (8 weeks, 3 sessions per week, 30 mg / kg body weight). SOD activity of the muscle was measured using ELISA kits and serum MDA levels using Tobartic acid (TBARS) method. Data were analyzed using one-way ANOVA (ANOVA).

Results: The antioxidant enzyme activity of SOD in the endometrial muscle of endurance group (1.08 ± 0.222 μg / ml) was significantly lower than control group (22.2 ± 0.481 kg) (P = 0.043), and SOD activity in the endurance + resistance group (1.87 ± 0.172, p = 0.44), endurance + curcumin (2.24 ± 0.222; P = 0.039), and endurance + curcumin + resistance (0.202 ± 0.15, p = 0.029) was significantly higher than endurance group. The levels of malondialdehyde in the endurance group (4.27 ± 0.438 nmol / ml protein) were significantly higher in comparison with the control group (3.42 ± 0.350) (0.331) and Also, serum MDA levels in endurance + resistance groups (± 3.03 ± 0.342, p = 0.003), endurance + curcumin (p = 0.001, p <0.001), and endurance + curcumin + Resistance (3.32 ± 0.349, p = 0.008) was significantly lower than endurance group.

Conclusion: According to the findings, curcumin supplementation and light resistance training alone (curcumin: SOD increased by 35% and MDA decreased by 50%, resistance training: SOD increased by 18% and MDA decreased by 40%) And the combination of these (SOD 39% increase and MDA 30% reduction) prevents severe oxidative stress caused by endurance training. Therefore, it is advisable for athletes who use endurance training in their practice programs to use these two supplements.