[Home ] [Archive]   [ فارسی ]  
:: Main :: About :: Current Issue :: Archive :: Search :: Submit :: Contact ::
:: Volume 18, Issue 8 (5-2013) ::
__Armaghane Danesh__ 2013, 18(8): 590-602 Back to browse issues page
In Vitro Maturation and Embryo Development to blastocyst Mouse Germinal Vesicle Oocytes after Vitrification
M Nikseresht , T Rasti , M Jafari Barmak , H Ghasemi Hamidabadi , Z Rezaei , F Dehghani , R Mahmoudi 1
1- , rmahmoudi40@yahoo.com
Abstract:   (2672 Views)
Abstract Background & aim: Vitrification is a simple and ultra rapid technique for the conservation of fertility. Improving pregnancy rate associate with the use of cryopreserved oocytes would be an important advanced in human assisted reproductive technology (ART). The purpose of this study was to evaluate survival, oocytes maturation and embryo development to the blastocyst stage after vitrification of oocytes germinal vesicle-stage and multi stage Methods: In the present experimental study, germinal vesicle oocytes with or without cumulus cells were transferred to vitrification solution containing 30% (v/v) ethylene glycol, 18% (w/v) Ficoll-70, and 0.3 M sucrose, either by single step or in a step-wise way. After vitrification and storage in liquid nitrogen, the oocytes were thawed and washed twice in culture medium TCM119, and then subjected to in vitro maturation, fertilization, and culture. Data analysis was performed by using One-way variance and Tukey tests. Results: Oocytes survival, metaphase 2 stage oocyte maturation, fertilization and embryo formed blastocyst in vitrification methods multistage were significantly higher than the single step procedure (P<0/05) Conclusion: The Germinal vesicle stage oocytes vitrified with cumulus cells and stepwise procedure had positive effect on the survival, maturation and developmental rate on blastocyst compared to oocytes without cumulus cell and single step procedure. Key words: Germinal Vesicle Oocyte, Blastocyst, Vitrification, Ethylene glycol
Keywords: Key words: Germinal Vesicle Oocyte, Blastocyst, Vitrification, Ethylene glycol
Full-Text [PDF 553 kb]   (695 Downloads)    
Type of Study: Research | Subject: Special
Received: 2015/05/4 | Accepted: 2015/05/4 | Published: 2015/05/4
Send email to the article author

Add your comments about this article
Your username or Email:

CAPTCHA code


XML   Persian Abstract   Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Nikseresht M, Rasti T, Jafari Barmak M, Ghasemi Hamidabadi H, Rezaei Z, Dehghani F et al . In Vitro Maturation and Embryo Development to blastocyst Mouse Germinal Vesicle Oocytes after Vitrification. Armaghane danesh. 2013; 18 (8) :590-602
URL: http://armaghanj.yums.ac.ir/article-1-447-en.html


Volume 18, Issue 8 (5-2013) Back to browse issues page
ارمغان دانش Armaghane danesh
Persian site map - English site map - Created in 0.05 seconds with 30 queries by YEKTAWEB 3790