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:: Volume 18, Issue 8 (5-2013) ::
__Armaghane Danesh__ 2013, 18(8): 590-602 Back to browse issues page
In Vitro Maturation and Embryo Development to blastocyst Mouse Germinal Vesicle Oocytes after Vitrification
M Nikseresht , T Rasti , M Jafari Barmak , H Ghasemi Hamidabadi , Z Rezaei , F Dehghani , R Mahmoudi 1
1- , rmahmoudi40@yahoo.com
Abstract:   (6000 Views)
Abstract Background & aim: Vitrification is a simple and ultra rapid technique for the conservation of fertility. Improving pregnancy rate associate with the use of cryopreserved oocytes would be an important advanced in human assisted reproductive technology (ART). The purpose of this study was to evaluate survival, oocytes maturation and embryo development to the blastocyst stage after vitrification of oocytes germinal vesicle-stage and multi stage Methods: In the present experimental study, germinal vesicle oocytes with or without cumulus cells were transferred to vitrification solution containing 30% (v/v) ethylene glycol, 18% (w/v) Ficoll-70, and 0.3 M sucrose, either by single step or in a step-wise way. After vitrification and storage in liquid nitrogen, the oocytes were thawed and washed twice in culture medium TCM119, and then subjected to in vitro maturation, fertilization, and culture. Data analysis was performed by using One-way variance and Tukey tests. Results: Oocytes survival, metaphase 2 stage oocyte maturation, fertilization and embryo formed blastocyst in vitrification methods multistage were significantly higher than the single step procedure (P<0/05) Conclusion: The Germinal vesicle stage oocytes vitrified with cumulus cells and stepwise procedure had positive effect on the survival, maturation and developmental rate on blastocyst compared to oocytes without cumulus cell and single step procedure. Key words: Germinal Vesicle Oocyte, Blastocyst, Vitrification, Ethylene glycol
Keywords: Key words: Germinal Vesicle Oocyte, Blastocyst, Vitrification, Ethylene glycol
Full-Text [PDF 553 kb]   (1710 Downloads)    
Type of Study: Research | Subject: Special
Received: 2015/05/4 | Accepted: 2015/05/4 | Published: 2015/05/4
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Nikseresht M, Rasti T, Jafari Barmak M, Ghasemi Hamidabadi H, Rezaei Z, Dehghani F et al . In Vitro Maturation and Embryo Development to blastocyst Mouse Germinal Vesicle Oocytes after Vitrification. armaghanj 2013; 18 (8) :590-602
URL: http://armaghanj.yums.ac.ir/article-1-447-en.html


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Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Volume 18, Issue 8 (5-2013) Back to browse issues page
ارمغان دانش Armaghane Danesh
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