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:: Volume 21, Issue 2 (5-2016) ::
__Armaghane Danesh__ 2016, 21(2): 135-147 Back to browse issues page
The Effect of Hydro-alcoholic Extract of Hyperricum PerforatumL. on Some Blood Parameters in Male Rats Treated with Cyclophosphamide
L Yaghobi1, N Mirazi 2
1- Department of Biology, Faculty of Basic Sciences, Bu-Ali Sina University, Hamedan, Iran
2- Department of Biology, Faculty of Basic Sciences, Bu-Ali Sina University, Hamedan, Iran , mirazi205@gmail.com
Abstract:   (2349 Views)

Background & aim: Cyclophosphamide is an anti cancer drug which causes alkylation of DNA in cells. The side effects of cyclophosphamide are bone marrow damages and anemia. Hypericum perforatum is a medicinal plant which widely used in traditional medicine. In this study the hemotopoetic effect of Hypericum perforatum leaf extract (HPE) on bone marrow function and blood parameters in male rat were treated with cyclophosphamide was investigated.

Methods: Forty two male Wistar rats with an average body weight of 220±25 gr were randomly divided into 6 groups (n=7): control group taking normal saline,0.5ml/day, i,p ),witness group taking cyclophosphamide (15mg/kg/day, i.p.), positive control group taking HPE (200mg/kg, i.p/day),  treated groups 1, 2 and 3 recieved cyclophosphamide ,(15mg/kg/day, i.p. + 100mg/Kg, 200mg/Kg and 400mg/kg  HPE /day for 10 days, i,p). At the end of study blood samples were collected from heart directly. RBC, WBC, Hct, Hb and PL were meseaured. Data are presented as mean ± standard deviation and were analyzed using ANOVA and Tukey tests.

Results: The results indicated that cyclophosphamide caused damage on bone marrow, which in this case significantly reduced the number of blood cells and platelets in the group treated with cyclophosphamide (P<0.001). Blood parameters in the groups treated with HPE increased significantly compared with the group which received cyclophosphamide alone (P<0.001).

Conclusion: The Hypericum perforatum hydoethanolic extract contains antioxidant and flavonoids compounds which could protect the bone marrow tissues against cyclophosphamide.     

 

Keywords: Hypericum perforatum, Blood parameters, Cyclophosphamide, Rat
Full-Text [PDF 520 kb]   (699 Downloads)    
Type of Study: Research | Subject: Physiology
Received: 2015/07/11 | Accepted: 2016/04/23 | Published: 2016/05/9
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yaghobi L, mirazi N. The Effect of Hydro-alcoholic Extract of Hyperricum PerforatumL. on Some Blood Parameters in Male Rats Treated with Cyclophosphamide. Armaghane danesh. 2016; 21 (2) :135-147
URL: http://armaghanj.yums.ac.ir/article-1-960-en.html


Volume 21, Issue 2 (5-2016) Back to browse issues page
ارمغان دانش Armaghane danesh
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