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:: Volume 20, Issue 11 (2-2016) ::
__Armaghane Danesh__ 2016, 20(11): 1011-1023 Back to browse issues page
Investigation of Interleukin-17 gene polymorphism on DAP-Kinase gene promoter methylation in Patients with Breast Cancer
S Naeimi
Islamic Azad university,kazeron,iran , naeimis@kau.ac.ir
Abstract:   (3311 Views)

Background & Aim: Breast cancer is the most common malignancy in women . Studies have shown that increased in methylation of CpG islands (CpG island hyper methylation, CIHM), is one of the important mechanisms in gene down regulation. DAP-Kinase protein plays an important role in the process of Apoptosis. Interleukin-17 is an proinflammatory cytokine and inflammation,is one of the factors  that affect on gene methylation . the purpose of this study was to evaluate the polymorphism of the IL-17 gene promoter methylation Dap-kinase and its relationship to breast cancer.

Methods: In this case - control study, A total of 40 Women with Breast cancer and 40 healthy women in Iran were examined.DNA was extracted by saluting out method and Single nucleotide Polymorphisms of the IL-17 gene were analyzed by the PCR-RFLP method and To study gene promoter methylation Dap-kinase, MSPCR method was used.data were compared in both groups by using Pearson’s chi-square and Hardy-weinberg equilibrium test.

RESULTS: Results confirm the fact that, there is a relationship between DAP-kinase gene promoter methylation and breast cancer disease So that the promoter of this gene in patients than in healthy individuals was much more methylated( p<0.05) . On the other hand there is no significant coloration between IL-17 gene polymorphisms and DAP-kinase gene methylation (P>0.05)

Conclusion: Due to the fact, that promoter genes methylation is one of the mechanisms of epigenetic genes silencing, it seems that DAP-kinase gene promoter methylation increases is associated with the risk of breast cancer in women.

Keywords: Polymorphism, Breast Cancer, IL-17, Methylation, DAP-Kinase
Full-Text [PDF 285 kb]   (1054 Downloads)    
Type of Study: Research | Subject: Special
Received: 2015/06/23 | Accepted: 2016/01/15 | Published: 2016/01/23
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Naeimi S. Investigation of Interleukin-17 gene polymorphism on DAP-Kinase gene promoter methylation in Patients with Breast Cancer. Armaghane danesh. 2016; 20 (11) :1011-1023
URL: http://armaghanj.yums.ac.ir/article-1-913-en.html

Volume 20, Issue 11 (2-2016) Back to browse issues page
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