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Prevalence of the cagA-Positive Helicobacter pylori Strains Isolated from Gastric Biopsy Specimens in Shahrekord
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A Doosti1   , GA Rahimian , J Nassiri , P Yavari-Forushani |
| 1- , doostiiranii@yahoo.com |
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Abstract: (4145 Views) |
| ABSTRACT:
Introduction & Objective: Helicobacter pylori are among
the important pathogens responsible for chronic gastritis,
peptic ulcer and gastric cancer. The present study aimed to
comparatively evaluate PCR and RUT methods for detection
of H. Pylori and determination of the prevalence of the cagApositive
Helicobacter pylori strains in Shahrekord.
Materials & Methods: This is a molecular epidemiology study
conducted in 1385 on biopsy samples collected from 120 patients
with dyspeptic symptoms who were referred to endoscopy
department of Hajar hospital of Shahrekord. In order to detect H.
pylori, RUT method was used at first and then DNA was directly
extracted from biopsy specimens. PCR-amplification was
preformed for the ureC and then for cagA gene.
Results: The H. pylori infection was found in 74 (61.66%) of the
patient by RUT method. In parallel, ureC PCR detected H. pylori in
103 (85.83%) of patients. All RUT-positive patients were found to
be ureC-PCR positive, too. The cagA-positive H. pylori strains
were found in 83.5 percent of isolated strains.
Conclusion: These findings indicate that ureC PCR is more
sensitive than RUT for diagnosis of H. pylori infection. In addition,
the high prevalence of the CagA-positive H. pylori strains is
present in Shahrekord. |
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| Keywords: Helicobacter pylori, Polymerase Chain Reaction(PCR), Cytotoxin associated gene A(cagA) |
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Full-Text [PDF 222 kb]
(1225 Downloads)
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Type of Study: Research |
Subject:
Special
Received: 2015/06/15 | Accepted: 2015/06/15 | Published: 2015/06/15
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