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:: Volume 19, Number 2 (5-2014) ::
__Armaghane Danesh__ 2014, 19(2): 124-135 Back to browse issues page
Evaluation of Protective Effects of Crocin Onembyo Developing Process in in Vitro Fertilization (IVF)in Cyclophosphamide Treated Mice
F Khan Mohammadi , R Shahrooz, A Ahmadi, M Razi
Abstract:   (1072 Views)
Abstract Background & aim: Cyclophosphamide (CP) along with therapeutic role, exert the effects such as an immunosuppressant agent, induce oxidative stress, impact gonadal cell’s DNA and reducing the fertilizing potential. The present study was aimed to evaluate the protective effects of crocin, on IVF embryos development following chemotherapy with CP. Methods: In this study 36 adult female mice, aged 6-8 weeks, divided into 3 groups and treared for 21 days. The control animals received saline normal (0.1ml/day, IP), and sham control group received CP alone (15 mg/kg/week, IP) and experimental group received crocin (200mg/kg/day, IP) along with CP (15 mg/kg/week, IP). After the end of treatment period PMSG and HCG were administrated for stimulating the ovulation process. The sperms were obtained from 6 adult male mice. Following the euthanizing of animals, and oocyte along with normal sperm collection in-vitro fertilizing was performed by using HTF+4mg/ml BSA medium. The fertilized oocytes were incubated for 120 hours and embryos were studied in various stages. All data were analyzed statistically by Minitab software. Results: In sham control group a significantly reduction in suitable oocytes, percentage of fertilization, two cells zygote, blastocysts and increase the number of arrested developing embryos observed in comparison to control group, and administration of crocin caused significantly increase in suitable oocytes, percentage of fertilization, two cells zygote, blastocysts and significantly reduction of arrested developing embryos in comparison to sham control group (P<0.05). Conclusion: The co-administration of crocin with CP chemotherapy caused a significant improvement in fertilizing potential and promoted the embryo development. Key words: Cyclophosphamide, Crocin, Mice, Oocyte, In vitro fertilizing
Keywords: Key words: Cyclophosphamide, Crocin, Mice, Oocyte, In vitro fertilizing
Full-Text [PDF 450 kb]   (319 Downloads)    
Type of Study: Research | Subject: Special
Received: 2015/05/4 | Accepted: 2015/05/4 | Published: 2015/05/4
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Khan Mohammadi F, Shahrooz R, Ahmadi A, Razi M. Evaluation of Protective Effects of Crocin Onembyo Developing Process in in Vitro Fertilization (IVF)in Cyclophosphamide Treated Mice. Armaghane danesh. 2014; 19 (2) :124-135
URL: http://armaghanj.yums.ac.ir/article-1-441-en.html
Volume 19, Number 2 (5-2014) Back to browse issues page
ارمغان دانش Armaghane danesh
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