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:: Volume 20, Issue 2 (5-2015) ::
__Armaghane Danesh__ 2015, 20(2): 89-102 Back to browse issues page
Evaluation of the Genetic Variation of Non Coding Control Region of BK Virus Using Nested-PCR Sequencing Method in Renal Graft Patients
A Emami1, R Yaghobi 2, A Moattari3, M Baseri salehi4, J Roozbeh5
1- Department of Microbiology, Science and research branch, Islamic Azad University, Fars, Iran
2- Shiraz Transplant Research Center, Shiraz University of Medical Sciences, Shiraz, Iran , rayaviro@yahoo.com
3- Department of Bacteriology and Virology, Shiraz University of Medical Sciences, Shiraz, Iran
4- Department of Microbiology, Kazeroon branch, Islamic Azad University, Kazeroon, Iran,
5- Shiraz Nephrology Urology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran
Abstract:   (3796 Views)

Background & aim: Polyomaviruses (BK) is a comprehensive infection with more than of 80% prevalence in the world. One of the most important reasons of BK virus nephropathy is in the renal transplant recipients and rejection of transplanted tissue between them. Non Coding region of this virus play a regulatory role in replication and amplification of the virus. The aim of this study was to evaluate the genetic patterns of this area in renal graft at Namazi Transplantation Center, Shiraz, Iran. Methods: In the present experimental study, 380 renal allograft serums were collected. DNAs of 129 eligible samples were extracted and evaluated using a virus genome. The presence of the virus was determined by qualitative and sequencing. Of these, 129 samples were tested for the presence of virus according to the condition study, using quantitative, qualitative genomic amplification and sequencing. Results: The study showed symptoms of nephropathy, 76 (58.9%) of them were males and 46 (35.7%) were females with the mean age 38.0±.089 years of age. In general, 46 patients (35.7%) percent) were positive for BK Polyomaviruses. After comparing the genomic sequence with applications of molecular they were categorized in three groups and then recorded in gene bank. Conclusion: About 35% of renal transplant recipients with high creatinine levels were positive for the presence of BK virus. Non-coding region of respondents in the sample survey revealed that among patients with the most common genotypes were rearranged the entire transplant patients were observed at this tranplant center. Examination of these sequences indicated that this rearrangments had a specific pattern, different from the standard strain of archaea type.

Keywords: Polyomavirus BK, Renal graft, Nephropathy, Non Coding Control Region, Shiraz
Full-Text [PDF 682 kb]   (1081 Downloads)    
Type of Study: Research | Subject: Special
Received: 2015/05/4 | Accepted: 2015/05/4 | Published: 2015/05/4
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Emami A, Yaghobi R, Moattari A, Baseri salehi M, Roozbeh J. Evaluation of the Genetic Variation of Non Coding Control Region of BK Virus Using Nested-PCR Sequencing Method in Renal Graft Patients. Armaghane danesh. 2015; 20 (2) :89-102
URL: http://armaghanj.yums.ac.ir/article-1-439-en.html


Volume 20, Issue 2 (5-2015) Back to browse issues page
ارمغان دانش Armaghane danesh
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