[Home ] [Archive]   [ فارسی ]  
:: Main :: About :: Current Issue :: Archive :: Search :: Submit :: Contact ::
:: Volume 17, Issue 5 (12-2012) ::
__Armaghane Danesh__ 2012, 17(5): 407-415 Back to browse issues page
Genotyping of Hepatitis B Virus in HBsAg Positive Individuals Referred to the Health Centers of Shahrekord, Iran
A Karimi , L Salimzadeh, N Bagheri, K Heidari, SM Hossein
Abstract:   (3007 Views)
Abstract Background & aim: Hepatitis B Virus is one of the most important viral hepatitis which includes eight genotypes based on genetic variations in the gene encoding virus RNA polymerase. Clinical picture, treatment response and prognosis of HBV infection is genotype dependent. Therefore, this study was aimed to determine the HBV genotypes in HBsAg-positive individuals. . Methods: This experimental study was conducted on one hundred and sixteen HBsAg-positiveindividuals referred to the health centers of Shahrekord, Iran, in 2011. Firstly, the viral nucleic acid was extracted from serum samples and subsequently, the samples were subjected to Polymerase Chain Reaction (PCR). Finally, genotyping was carried out on the positive samples, using Real-time PCR with type specific primers and probes. The data were analyzed using the chi-square test. Results: 23 out of 116 (19.8%) of the HBsAg-positive individuals were positive for HBV DNA. 17 out of 23 (73.9%) and 6 out of 23 (26.1%) of the patients were found to be infected with HBV genotypes of D and C, respectively. Conclusion: Same as other regions of Iran, genotype D, , is the dominate genotype of HBV in Shahrekord, Iran. However, genotype C may be one of the other common genotypes in this region. Key words: Genotyping, Hepatitis, Polymerase Chain Reaction
Keywords: Key words: Genotyping, Hepatitis, Polymerase Chain Reaction
Full-Text [PDF 708 kb]   (737 Downloads)    
Type of Study: Research | Subject: Special
Received: 2015/04/29 | Accepted: 2015/04/29 | Published: 2015/04/29
Add your comments about this article
Your username or Email:

CAPTCHA


XML   Persian Abstract   Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Karimi A, Salimzadeh L, Bagheri N, Heidari K, Hossein S. Genotyping of Hepatitis B Virus in HBsAg Positive Individuals Referred to the Health Centers of Shahrekord, Iran. Armaghane danesh. 2012; 17 (5) :407-415
URL: http://armaghanj.yums.ac.ir/article-1-412-en.html


Volume 17, Issue 5 (12-2012) Back to browse issues page
ارمغان دانش Armaghane danesh
Persian site map - English site map - Created in 0.06 seconds with 32 queries by YEKTAWEB 4162