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:: Volume 20, Issue 3 (6-2015) ::
__Armaghane Danesh__ 2015, 20(3): 184-197 Back to browse issues page
Comparison of viability of adipose-derived Mesenchymal stem cells on agarose and fibrin glue scaffolds
Farzaneh Tafvizi1, Nasim Hayati roodbari2
1- Department of Biology, Parand Branch, Islamic Azad University, Parand, Iran , farzanehtafvizi54@gmail.com
2- Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran
Abstract:   (6270 Views)

Background & aim: Utilizing tissue engineering techniques and designing similar structures of the damaged tissues require the use of tools such as scaffolds, cells, and bioactive molecules in vitro. Meanwhile, appropriate cell cultures with the ability to divide and differentiate on the natural scaffolds lacking features like immunogenicity and tumorgenesis is particularly important. Adipose tissue has attracted researchers’ attention due to its abundance of mesenchymal stem cells and its availability through a liposuction. The purpose of the present study was to investigate the reproducibility and viability of the adipose-derived stem cells on natural scaffolds of fibrin glue and agarose. Methods: In the present experimental study, the isolation and identification of the mesenchymal stem cells was performed on tissue obtained from liposuction. The tissues were extensively washed with PBS and were digested with collagenase I, then the mesenchymal stem cells were isolated. The cells were cultured in RPMI medium supplemented with antibiotic. Subsequently, the expression of cell surface markers including CD34, CD44, CD90, and CD105 were analyzed by flow cytometry to confirm the mesenchymal cells. After preparing fibrin glue and agarose scaffolds, the viability and proliferation of the adipose tissue-derived mesenchymal stem cells were examined at the period of 24, 48, and 72 hours by MTT and ELISA assays. The obtained results were analyzed by SPSS ver.19. Results: The results of adipose tissue-derived mesenchymal stem cells culture on the fibrin glue and agarose scaffolds indicated that cell viability on fibrin glue and agarose scaffold were 68.22% and 89.75% in 24 hrs, 64.04% and 66.97% in 48 hours, 222.87% and 1089.68% in 72 hours respectively. Significant proliferation and viability cells on a synthesized agarose scaffold were seen compared to the fibrin glue scaffold after 72 hrs. The viability of the cells significantly increased on the agarose scaffold. Conclusion: Due to stability and permeability of scaffolding agarose, it seems that scaffolding agarose created better adhesion of cells in the performance of cell proliferation process compared with fibrin glue scaffold.

Keywords: Adipose, derived mesenchymal stem cells, Agarose scaffold, Fibrin glue scaffold, MTT assay
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Type of Study: Research | Subject: Special
Received: 2015/01/28 | Accepted: 2015/04/28 | Published: 2015/05/31
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Tafvizi F, hayati roodbari N. Comparison of viability of adipose-derived Mesenchymal stem cells on agarose and fibrin glue scaffolds . ارمغان دانش. 2015; 20 (3) :184-197
URL: http://armaghanj.yums.ac.ir/article-1-39-en.html

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Volume 20, Issue 3 (6-2015) Back to browse issues page
ارمغان دانش Armaghane Danesh
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