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:: Volume 30, Issue 2 (2-2025) ::
__Armaghane Danesh__ 2025, 30(2): 203-216 Back to browse issues page
Enhancement of the Anticancer Effect of Paclitaxel on Human Ovarian Cancer SKOV-3 and A2780s Cell Lines with the Help of Rapamycin as a Kinase Inhibitor
B Karimi1 , H Bardania2 , R Mahmoudi2 , M Tajali2 , H Abidi2 , M Dehghani3 , M Nikseresht4
1- Student Research Committee, Yasuj University of Medical Sciences, Yasuj, Iran,
2- Cellular and Molecular Research Center, Yasuj University of Medical Sciences, Yasuj, Iran
3- Hematology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran
4- Cellular and Molecular Research Center, Yasuj University of Medical Sciences, Yasuj, Iran , nikmohsen65@gmail.com
Abstract:   (576 Views)
Background & aim: Ovarian cancer is considered the most lethal type of cancer among female malignancies due to late diagnosis. Conventional treatment of ovarian cancer is surgical removal of the tumor mass followed by chemotherapy. Paclitaxel is considered a common therapeutic factor in ovarian cancer chemotherapy. Various studies have shown that paclitaxel induces drug resistance by activating or increasing the expression of the PI3K/AKT/mTOR signaling pathway. Therefore, the aim of the present study was to improve the anticancer effect of paclitaxel on human ovarian cancer cell lines SKOV-3 and A2780s with the help of rapamycin as a kinase inhibitor.

Methods: The present experimental in-vitro study was conducted in 2019. The ovarian cancer cell lines SKOV-3 and A2780s were cultured in RPMI-1640 medium supplemented with 10% fetal bovine serum, 100 μg/mL streptomycin, and 100 units/mL penicillin under controlled conditions of 37°C and a humidified atmosphere containing 5% carbon dioxide. The cytotoxic effect of paclitaxel and rapamycin was evaluated by MTT assay. The type of cell death was examined by flow cytometry. The collected data were analyzed using variance and t-test statistical tests using SPSS version 20 software.

Results: The results of MTT assay and flow cytometry analysis indicated that paclitaxel and rapamycin drugs reduced cell survival and induced cell death in SKOV-3 and A2780s ovarian cancer lines, and their effects were time and concentration dependent. The results of cytotoxicity assessment showed that when paclitaxel is used together with rapamycin, it causes 50% cell death at a lower concentration compared to when it is used alone, and their cytotoxic effect is significantly increased (p<0.001).

Conclusion: Rapamycin increased the sensitivity of ovarian cancer cells to paclitaxel by reducing the expression of p70s6k and mTOR genes involved in the PI3K/AKT/mTOR signaling pathway, which was involved in cell growth and proliferation and many cell functions. This could be a useful and good therapeutic strategy for the treatment of ovarian cancer.

 
Keywords: Ovarian cancer, Paclitaxel, SKOV-3, A2780s, Rapamycin
Full-Text [PDF 582 kb]   (94 Downloads)    
Type of Study: Research | Subject: Biochemistry
Received: 2025/01/20 | Accepted: 2025/05/20 | Published: 2025/09/14
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Karimi B, Bardania H, Mahmoudi R, Tajali M, Abidi H, Dehghani M et al . Enhancement of the Anticancer Effect of Paclitaxel on Human Ovarian Cancer SKOV-3 and A2780s Cell Lines with the Help of Rapamycin as a Kinase Inhibitor. armaghanj 2025; 30 (2) :203-216
URL: http://armaghanj.yums.ac.ir/article-1-3788-en.html


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Volume 30, Issue 2 (2-2025) Back to browse issues page
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