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The Expression Levels and Significance of GSH, MDA, SOD, and 8-OHdG in the Induced Osteoarthritis in Rabbit Knee Joints
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Hossein Maghsoudi1   , Fereshte Joulla2 , Gholamreza Bakhshi Khanaki2 |
1- Payame Nour University , hossein_m2002@yahoo.com
2- Payame Nour University |
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Abstract: (54 Views) |
Background & aim: Oxidative stress is caused by an imbalance between the oxidative and antioxidant systems of cells and tissues and, as a result, the excessive production of oxidative free radicals and reactive oxygen species associated with free radicals. The relationship between oxidative stress caused by the slow production of endogenous free radicals and osteochondral defects has been confirmed by a large number of researchers. Studies have shown that excessive production of free radicals causes osteochondrocyte apoptosis and extracellular matrix degradation, which ultimately causes osteochondral defects. The main biomarkers of oxidative stress are: glutathione, malondialdehyde, superoxide dismutase and 8-hydroxydeoxyguanosine. The aim of this study was to observe dynamic changes in oxidative stress and the expression levels of antioxidant and oxidative parameters in blood and synovial fluid in osteoarthritis-induced knee joints of rabbits.
Method: Thirty New Zealand White rabbits were randomly selected and divided into control group (healthy rabbits) (10 heads), treatment group one (rabbits with treated osteoarthritis) (10 heads), and treatment group two (rabbits with untreated osteoarthritis) (10 heads). The rabbits were kept for 1 month to adapt to the laboratory conditions. Collagenase was injected intra-articularly into the right knees of the rabbits. 30 days after the development of collagenase-induced osteoarthritis, the expression levels of glutathione, malondialdehyde, superoxide dismutase, and 8-hydroxydeoxyguanosine in peripheral venous blood and knee synovial fluid in the three groups were measured at the end of 4. 8 and 12 weeks after treatment.
Result: The expression levels of glutathione and superoxide dismutase in the blood and synovial fluid of the second treatment group at the end of weeks 8 and 12 were significantly lower than the other two groups (P < 0.05). Higher expression levels of malondialdehyde and 8-hydroxydeoxyguanosine were obtained in the blood and synovial fluid of the second treatment group compared to the other two groups. (P < 0.05) At the end of weeks 4, 8 and 12, the expression levels of malondialdehyde and 8-hydroxydeoxyguanosine in the blood and synovial fluid of the positive test group showed an upward trend (P < 0.05)
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Conclusion: Osteochondral defects initiate oxidative stress in the body, which is manifested as decreased expression of glutathione and superoxide dismutase and upregulation of malondialdehyde and 8-hydroxydeoxyguanosine expression.
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| Keywords: malondialdehyde, superoxide dismutase, 8-hydroxydeoxyguanosine, Glutathione |
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Type of Study: Research |
Subject:
Clinical laboratory
Received: 2025/02/10 | Accepted: 2025/09/28 | Published: 2025/10/18
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