1- Department of Microbiology, Falavarjan Branch, Islamic Azad University, Isfahan, Iran 2- Department of Microbiology, Falavarjan Branch, Islamic Azad University, Isfahan, Iran , ghandeharifereshte@gmail.com 3- Department of Biology, School of Basic Sciences, Boroujerd Azad University, Boroujerd, Iran
Abstract: (1644 Views)
Background & aim:One of the most important challenges today is the spread of methicillin and vancomycin resistant strains of Staphylococcus aureus. Acquisition of antibiotic resistance and changes in staphylococcal pathogenicity patterns are the most important causes of virulence. Therefore, the aim of the present study was to determine and identify the pattern of antibiotic resistance in methicillin and vancomycin resistant strains of Staphylococcus aureus isolated from clinical samples of metropolitan hospitals in Tehran. Methods: In the present cross-sectional descriptive study conducted in 2018, 502 clinical specimens of Staphylococcus aureus were identified and confirmed by biochemical tests and amplification of s_rRNA16 gene by PCR. All isolates were tested for resistance to methicillin by disk method and for resistance to vancomycin using the least inhibitory concentration method. Finally, both groups were tested for molecular weight. The collected data were analyzed using one-way analysis of variance and error coefficient less than 0.05% using statistical tests. Results: Out of 502 isolates, 168 isolates were resistant to methicillin (33.46%), 6 isolates were resistant to vancomycin (1.19%) and 2 isolates had intermediate resistance to vancomycin (0.39%). The frequency of mecA gene in isolates Methicillin resistance was 98.8%, vanB and vanC1 gene frequency was 37.5% each and vanC3 gene frequency was 12.5% in vancomycin resistant isolates. The highest resistance of methicillin-resistant isolates was isolated from the catheter and the highest resistance of vancomycin-resistant isolates with intermediate resistance to vancomycin were isolated from the secretion site. In both groups, the highest resistance was related to adults. Conclusion: VRSA and MRSA are considered as a serious threat for human health. Correct detection of the infection resistance patterns and the use of convenient antibiotic can decrease the speed of the development of bacteria resistance.
Eslamnezhad N, Ghandehari F, Mirzaee M, Madani M, Mehrabi M. Antibiotic Resistance Patterns of Methicillin and Vancomycin -Resistant Staphylococcus Aureus Isolated from Tehran Hospitals' Clinical Samples. armaghanj 2021; 26 (5) :793-814 URL: http://armaghanj.yums.ac.ir/article-1-3083-en.html