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Evaluation of Protective Effects of Hydroalcoholic Extract Obtained from Cucurbita moschata Skin in Animal Model of Ulcerative Colitis
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N Fitras1   , GH Bahrami2 , KH Rashidi2 , M Kiarostami1 , MH Farzaei  3 |
1- Student Research Committee, Kermanshah University of Medical Sciences, Kermanshah, Iran
2- Adipose tissue and Oils Research Center, Health Technology Research Institute, Kermanshah University of Medical Sciences, Kermanshah, Iran
3- Pharmaceutical Sciences Research Center, Health Research Institute, Kermanshah University of Medical Sciences, Kermanshah, Iran , mh.farzaei@gmail.com |
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Abstract: (1062 Views) |
Background & aim: Ulcerative colitis is an inflammatory disease of the large intestine that cannot be treated, but its symptoms can be controlled. In traditional medicine, for the basic treatment of colitis, it is necessary to first prevent this abnormal substance from leaking into the intestines. Pumpkin, scientifically known as Cucurbita moschata from the Cucurbitaceae family, has wound healing as well as anti-inflammatory properties. In this regard, the aim of the present study was to determine and evaluate the protective effects of hydroalcoholic extract prepared from the skin of the pumpkin plant in an animal model of ulcerative colitis.
Methods: In the present experimental study conducted in 2020, for extraction from the skin of the squash fruit, 150 g of the dried and crushed skin of the fruit was transferred to a glass percolator and using a solvent of water and ethanol in a ratio of 70 30: Extracted in three stages. 36 field rats were prepared and randomly divided into 6 groups (6 mice in each group). In order to induce colitis, 4% acetic acid was used to evaluate the effects of the extract obtained from pumpkin skin, this extract was administered by gavage in 3 doses for 6 days to mice. Finally, the studied mice were examined for macroscopic changes and histological studies. The collected data were analyzed using ANOVA statistical tests.
Results: In macroscopic examinations of the colon among different groups, in the healthy group the colon mucosa was completely normal. In the negative control group, side effects such as; Wounds, adhesions, thickening of the intestinal wall and severe inflammation were observed. In the groups receiving the extract in 3 doses, reduction of inflammation and wound healing was observed in mice with colitis, which showed the best results at a dose of 200 mg / kg. In the treatment of squash extract extract in a dose-dependent manner, it showed significant effects in inducing healing in acetic acid-induced colitis in mice (p<0.05).
Conclusion: Since the complications of colitis induction in the groups receiving pumpkin skin extract were significantly reduced compared to the control group, especially at a dose of 200 mg / kg, the results of this study can be groundbreaking. Clinical trial studies should be used in relation to ulcerative colitis and as a complementary drug to other treatments.
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| Keywords: Ulcerative colitis, Traditional medicine, Medicinal plants, Pumpkin |
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Full-Text [PDF 887 kb]
(319 Downloads)
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Type of Study: Research |
Subject:
Special
Received: 2021/02/1 | Accepted: 2021/11/13 | Published: 2022/02/28
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