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Indexing & Abstracting
:: Volume 17, Issue 3 (8-2012) ::
__Armaghane Danesh__ 2012, 17(3): 243-252 Back to browse issues page
Characterization of Isolated Hydatid Cyst from Slaughtered Livestock in Yasuj Industrial Slaughterhouse by PCR-RFLP
A Sadri , A Moshfe 1, A Doosti , H Ansari , H Abidi , S Ghorbani Dalini
1- , amoshfea@yahoo.com
Abstract:   (4496 Views)
Background & aim: Given the existence of 10 different genotypes of the parasite Echinococcus granulosus from different hosts and intermediate and final impact of these genotypes in the life cycle of the parasite and its transmission to humans, the purpose of this study was to determine the molecular characterization of isolates of hydatid cysts in industrial slaughterhouses of Yasuj city. Methods: In this study, 93 animal isolates (56 goat, 31 sheep and 6 cattle) were collected from the industrial slaughterhouse of Yasuj city. The genomic DNA corresponding to protoscolices was extracted, using the standard Phenol–Chloroform method. The fragment of DNA-ITSI of each sample was assessed by PCR with designed primers of EgF, EgR and then amplified. Moreover, the PCR products were assessed by electrophoresis and digested by the Alu I and Rsa I enzymes. RFLP products were evaluated by electrophoresis. Results: Using a PCR test, rDNA-ITSI of all isolates of similar size bands and 1000 bp were obtained respectively.The patterns generated by RFLP using Alu I and Rsa I enzymes, showed Echinococcus granulosus G1 genotype in all isolates. Conclusion: This study showed that strain G1 is the predominant strain causing hydatid cysts in different organs of the animal in Yasuj.
Keywords: Echinococcus granulosus, rDNA-ITSI, PCR-RFLP
Full-Text [PDF 611 kb]   (2197 Downloads)    
Type of Study: Research | Subject: Special
Received: 2015/04/19 | Accepted: 2015/04/19 | Published: 2015/04/19
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Sadri A, Moshfe A, Doosti A, Ansari H, Abidi H, Ghorbani Dalini S. Characterization of Isolated Hydatid Cyst from Slaughtered Livestock in Yasuj Industrial Slaughterhouse by PCR-RFLP. armaghanj 2012; 17 (3) :243-252
URL: http://armaghanj.yums.ac.ir/article-1-260-en.html

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Volume 17, Issue 3 (8-2012) Back to browse issues page
ارمغان دانش Armaghane Danesh
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