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:: Volume 24, Issue 4 (9-2019) ::
__Armaghane Danesh__ 2019, 24(4): 0-0 Back to browse issues page
Study effects of Staphylococcus aureus superantigen C on production of C- reactive protein in rat
Mohammad Reza Ataee1 , Gholam Hosien Alishiri 2, Ardeshir Hesampoor Mahalati1 , Ramezan Ali Ataee3
1- Azad UNversity
2- Baqiyatallah University of Medical Sciences , ghalishiri@gmail.com
3- Baqiyatallah University of Medical Sciences
Abstract:   (442 Views)
Background and purpose: Recently, microbial super antigens have been reported in blood and synovial fluid of rheumatoid arthritis patients. Whether or not, the presence of these super antigens could provoke the induction of serologic diagnostic factors, including CrP in patients? the purpose of this study was to production and purification one of the super antigen C of Staphylococcus aureus and its effect on the induction of CrP in rat
Materials and methods: Culture, Staphylococcus aureus super antigen C was purified by ultrafiltration (Amicon Ultra Centifugal Filter Device) and use specific antibody for immunoblotting confirmatory was carried out. The protein concentration was measured. 50 to 100 micrograms of toxin were injected intraperitoneal and intrasynovial into separate rat groups. In time course blood collection and CrP was tested. The results were analyzed.
Results: The results showed that 50 to 100 μg of toxin intra-articular and intraperitoneal after 20 days inducted CrP production at 0.7 mg. After 40 days, this increased to 2. 2 and after 50 days to 4.5 mg. ANOVA analysis was shown the difference between the groups and with a significant level for groups (P≤ 0.001) and for the intervals of the effect of super antigen (P≤ 0.075), respectively.
Conclusion: The results indicate that the presence and survival of super antigen C of in the body of rats induced the production of CrP factor. Although this finding is an introduction to further research, it is likely to provide new ways to prevent and control inflammatory diseases, including rheumatoid arthritis.
 
Keywords: purification, S. aureus, super antigen C, C-Reactive Protein and Rheumatoid arthritis
     
Type of Study: Research | Subject: Microbiology
Received: 2019/02/2 | Accepted: 2019/06/3 | Published: 2019/07/6
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Ataee M R, Alishiri G H, Hesampoor Mahalati A, Ataee R A. Study effects of Staphylococcus aureus superantigen C on production of C- reactive protein in rat. Armaghane danesh. 2019; 24 (4)
URL: http://armaghanj.yums.ac.ir/article-1-2437-en.html


Volume 24, Issue 4 (9-2019) Back to browse issues page
ارمغان دانش Armaghane danesh
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