[Home ] [Archive]   [ فارسی ]  
:: Main :: About :: Current Issue :: Archive :: Search :: Submit :: Contact ::
:: Volume 24, Issue 3 And ضمیمه1 (7-2019) ::
__Armaghane Danesh__ 2019, 24(3 And ضمیمه1): 461-473 Back to browse issues page
The Effect of High-Intensity Interval Training (HIIT) on Gene Expression of Apoptotic Markers in the Skeletal Muscle of Diabetic Rats
H Moradi1 , S Shadmehri 2, H Shirvani3
1- Department of Physical Education and Sport Science, Yadegare Imam Khomeini(RAH) Shahre-Rey Branch, Islamic Azad University, Tehran, Iran,
2- Department of Physical Education and Sport Science, Yadegare Imam Khomeini(RAH) Shahre-Rey Branch, Islamic Azad University, Tehran, Iran, , saeedehsh61@gmail.com
3- Exercise Physiology Research Center, Life Style Institute, Baqiyatallah University of Medical Sciences, Tehran, Iran
Abstract:   (608 Views)

Background & aim: Apoptosis plays an important role in the pathophysiology of Type 2 diabetes. The aim of this study was to evaluate the effect of high-intensity interval training (HIIT) on gene expression of apoptotic markers in the skeletal muscle of diabetic rats.

 

Methods: In the present experimental study, three male Wistar rats weighing 220±20 g were randomly divided into 4 groups including: baseline control, eight weeks control, diabetes mellitus, exercise and diabetes mellitus. In the present study, rats were diabetic intraperitoneally using streptozotocin in a single dose of 50 mg / kg. Intermittent exercise with intensity of 0.55% maximal oxygen consumption was performed 5 days a week for 2 weeks. The expression of Caspase3 and Bcl2 genes was measured by Ray-Tom-Pissar method. The data were analyzed using one-way ANOVA and Tukey post hoc tests.

 

Results: The results indicated that there was a significant difference between the mean of Bcl2 gene expression and Caspase-3 skeletal muscle in diabetic rats (p = 0.0001). Skeletal muscle Bcl2 gene expression was significantly higher in exercise (p = 0.0001) and exercise-diabetes (p = 0.048) than diabetic group (p = 0.0001). Furthermore, the expression of Caspase-3 gene in skeletal muscle was significantly lower in diabetic group (p = 0.0001).

 

Conclusion: Diabetes appears to be associated with increased skeletal muscle apoptosis, and vigorous intermittent exercise may help to regulate apoptosis in skeletal muscle during diabetes, therefore it is recommended that these exercises be considered for diabetic patients.

 

Keywords: High-intensity interval training, Caspas3, Bcl2, Diabetes
Full-Text [PDF 387 kb]   (65 Downloads)    
Type of Study: Research | Subject: Sport Physiology
Received: 2018/12/31 | Accepted: 2019/04/27 | Published: 2019/07/7
Send email to the article author

Add your comments about this article
Your username or Email:

CAPTCHA


XML   Persian Abstract   Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Moradi H, Shadmehri S, Shirvani H. The Effect of High-Intensity Interval Training (HIIT) on Gene Expression of Apoptotic Markers in the Skeletal Muscle of Diabetic Rats. Armaghane danesh. 2019; 24 (3) :461-473
URL: http://armaghanj.yums.ac.ir/article-1-2395-en.html


Volume 24, Issue 3 And ضمیمه1 (7-2019) Back to browse issues page
ارمغان دانش Armaghane danesh
Persian site map - English site map - Created in 0.07 seconds with 32 queries by YEKTAWEB 4056