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:: Volume 23, Issue 6 (1-2019) ::
__Armaghane Danesh__ 2019, 23(6): 765-776 Back to browse issues page
Association of polymorphism-31 G/C in the Survivin gene promoter with the risk of multiple sclerosis disease
Sahar Rezazadeh , Parisa Mohammadi Nejad 1
1- , sahar.rezazadeh@yahoo.com
Abstract:   (168 Views)
Background & Aim: Multiple Sclerosis (MS) is an immune system-dependent disease with unknown causes and is one of the most important neurological disabling diseases in adults, where the myelin section of the nervous system is destroyed. The link between MS and apoptosis in patients shows that the expression of many genes involved in inhibiting the immune cell apoptosis, such as Survivin gene, which causes more activity in these cells and increases the cerebrospinal fluid damage, has increased. The aim of this study was to investigate of Polymorphism-31 G/C in the Survivin gene promoter in patients with MS disease and to compare with healthy people and its association with the disease.
Methods: In the present case-control study, 100 Blood samples of MS patients in Alzahra Hospital Isfahan (Patient group) and 100 healthy blood samples were collected in Blood Transfusion Organization (Control group). After extraction of DNA by kit, of Survivin gene primer was used in the Polymerase Chain Reaction (PCR) method. Then, RFLP method was used to evaluate the polymorphism by EcoO109I restriction enzyme and electrophoresis.
Results: The amplification of Survivin gene with a length of 308 base pairs was observed on agarose gel. The EcoO109I restriction enzyme produced bands with lengths of 210 and 98 base pairs. A band of 308 base pairs, representing the genotype GG, lengths 210 and 98 base pairs, representing the genotype CC and all three lengths together, represent the GC genotype. After data analysis and validation, genotypic correlation of Polymorphism-31 G/C with the risk of MS disease, in CG genotype was P (0.330), OR (1.397) and CI (0.714-2.735) and also for CC genotype, it was P (0.532), OR (0.800) and CI (0.397-1.612).
Conclusion: In general, Survivin gene can be used for biomarker and polymorphism tests in MS and other diseases and by studying in larger populations and determining the frequency of alleles and genotypes, it is possible to better determine the role of this gene in future research is a good way to prevent the disease.
Keywords: Multiple Sclerosis, Polymorphism-31 G/C, Survivin gene, PCR RFLP
Full-Text [PDF 271 kb]   (9 Downloads)    
Type of Study: Research | Subject: Special
Received: 2018/08/25 | Accepted: 2018/12/30 | Published: 2018/12/30
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Rezazadeh S, Mohammadi Nejad P. Association of polymorphism-31 G/C in the Survivin gene promoter with the risk of multiple sclerosis disease. Armaghane danesh. 2019; 23 (6) :765-776
URL: http://armaghanj.yums.ac.ir/article-1-2279-en.html

Volume 23, Issue 6 (1-2019) Back to browse issues page
ارمغان دانش Armaghane danesh
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