Background & aim: Epstein-Barr virus is one of the major causes of Hodgkin's lymphoma present in the Rhodeshtrenberg cells, which is part of classical Hodgkin's lymphoma. TRADD gene is an essential adapter for complex signaling and induction of apoptosis, necrosis, activation of NF-kB and MAP Kinase. The present study aimed to examine the methylation pattern of the TRADD gene in patients with Hodgkin's lymphoma EBV positive.

Methods: The present study was a case-control one. The case group included 80 patients with Hodgkin's lymphoma and the control group was the tumor margin in the case group. Spectrophotometry and electrophoresis on agarose gel were used to determine the quantity and quality of DNA. A change in the pattern of methylation of the TRADD gene was carried out using the EvaGreen method and a comparison of the high resolution melting point with HRM method. The collected data were analyzed using ANOVA.

Results: In the present study, subtypes were respectively 46.3%, 25%, 11.3% ,10% and 7.5% nodular sclerosis, cellular solid, rich lymphocyte, nonspecific lymphocyte and unspecified. Comparison of the transformation pattern of hyperthyllation of TRADD gene in viral samples with non-viral samples was 61.3% and 32.5%, which indicated significant changes. Evaluation of the significance of TRADD methylation pattern by segmentation of lymphoma indicated a significant relation between T lymphoma, grade and different stages in presence of EBV virus (P=0.046).

Conclusion: The present study indicated that the methylation pattern of TRADD gene is closely related to EBV viral infections. EBV patients are highly affectd by change in the behavior of the TRADD gene, which results in malpractice of immune cells, and ultimately lymphoma.