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:: Volume 23, Issue 5 (11-2018) ::
__Armaghane Danesh__ 2018, 23(5): 0-0 Back to browse issues page
The study on frequency of ferredoxin gene mutation in Trichomonas vaginalis isolates from women with vaginitis referred to Yasuj gynecologic clinic in 2017
Abdolali Moshfe 1, Parvin Ghaffari 2 , Seyed sajjad Khoramrooz 2 , Tahere Bab 2 , Maral Ghareghani 2
1- yasuj university of medical sciences , amoshfea@yahoo.com
2- yasuj university of medical sciences
Abstract:   (63 Views)
Introduction: Trichomoniasis is the most common non-viral sexually transmitted disease worldwide. In previous studies in women referred to the Yasuj gynecologic clininc, Trichomonas vaginalis infection was reported by three methods: clinical diagnosis(19/04% - 28%), microscopic(42/9% - 41%) and polymerase chain reaction(11%). Parasite resistance to metronidazole is a therapeutic problem. In this resistance, mutation in the parasite ferredoxin gene plays an important role. In this study, frequency of mutation and determination point of the mutation in this gene, in isolated parasites from patients referring to Yasuj women's clinic in 2017 were investigated.
Material and Method: In this study, 46 patients with clinical signs of vaginitis were selected and after recording individual data with clinical symptoms in the questionnaire, two vaginal swab samples were prepared from each patient. One sample was placed in a test tube containing normal saline for microscopic examination and the other was placed in Dorset medium for growth the parasite. Finally, trophozoites DNA was extracted using a kit and two different PCR tests confirmed the presence of Trichomonas vaginalis and ferredoxin gene. The ferredoxin PCR genes products were sequenced by South Korea Bioneir Company and blast off at the NCBI base to find the location of the mutation. The results were analyzed using descriptive statistics methods.
Results: Out of 46 samples, 20 samples (43.44%) were positive for Trichomonas vaginalis in microscopic and culture media and 12 samples (20.08%) were positive in PCR. The ferredoxin gene was reproduced and sequenced in these 12 samples. No mutation in ferredoxin gene was detected in any of the studied samples.
Conclusion: Changes in the ferredoxin gene result in metronidazole discontinuation in the treatment of trichomoniasis, which was not seen in the study samples. This means that there is no therapeutic resistance associated with ferredoxin gene in these specimens.
Keywords: Trichomonas vaginalis, Ferredoxin gene, Vaginitis, Mutation
Type of Study: Research | Subject: Parasitology
Received: 2018/06/24 | Accepted: 2018/11/3 | Published: 2018/11/3
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moshfe A, ghaffari P, khoramrooz S S, bab T, ghareghani M. The study on frequency of ferredoxin gene mutation in Trichomonas vaginalis isolates from women with vaginitis referred to Yasuj gynecologic clinic in 2017. Armaghane danesh. 2018; 23 (5)
URL: http://armaghanj.yums.ac.ir/article-1-2230-en.html

Volume 23, Issue 5 (11-2018) Back to browse issues page
ارمغان دانش Armaghane danesh
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