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:: Volume 24, Issue 1 (4-2019) ::
__Armaghane Danesh__ 2019, 24(1): 17-30 Back to browse issues page
Apoptotic Effect of the Stachys pilifera Bent Plant Extracts on Colorectal Cancer Cell Line (HT- 29)
Esmaeel Panahi Kokhdan Mr , Hossein Sadeghi Dr , Hossein Ghafoori Dr , Heibatollah Sadeghi Profesor , Nazanin Danaei Ms , Mahmoud Reza Aghamaali Dr 1
1- , ar_asfaram@yu.ac.ir
Abstract:   (67 Views)
Background and aim: Stachys pilifera Benth (Lamiaceae) is used in traditional medicine to treat a variety of diseases. Despite some reports on the antitumor effects of some species of this genus, anticancer activity of Stachys pilifera has not been yet reported. Here, we examined the cytotoxic effect and cell death mechanisms of methanolic extract of Stachys pilifera and its alkaloid and terpenoid fractions on the HT-29 colorectal cell line.
Methods: HT-29 cells were cultivated and then incubated in the methanolic extract of Stachys pilifera and its fractions at various concentrations for 24 h. Cell viability was measured by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Effects of the tested extract and fractions were tested on some regulators of cell death and proliferation such as, DNA Fragmentation and Apoptosis by flowcytometry. Cisplatin was used as positive control. Data were analyzed by ANOVA and Tukey test
Results: The estimated inhibition concentration 50% values of the methanolic extract, alkaloid and terpenoid fractions, and cisplatin against HT-29 cell after 24 h were determined to,  Stachys pilifera extract and its fractions induced apoptosis through DNA fragmentation and apoptosis through flow cytometry Data showed considerable cytotoxic and antiproliferative effects of Stachys pilifera on colorectal cell line through induction of apoptosis.
Conclusion: These findings provide a basis for the therapeutic potential of S. pilfera in the treatment of colon cancer
 
Keywords: Stachys pilifera, apoptosis, DNA fragmentation and HT- 29
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Type of Study: Research | Subject: Biochemistry
Received: 2018/06/2 | Accepted: 2019/05/1 | Published: 2019/05/1
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Panahi Kokhdan E, Sadeghi H, Ghafoori H, Sadeghi H, Danaei N, Aghamaali M R. Apoptotic Effect of the Stachys pilifera Bent Plant Extracts on Colorectal Cancer Cell Line (HT- 29). Armaghane danesh. 2019; 24 (1) :17-30
URL: http://armaghanj.yums.ac.ir/article-1-2209-en.html


Volume 24, Issue 1 (4-2019) Back to browse issues page
ارمغان دانش Armaghane danesh
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