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:: Volume 23, Issue 3 (8-2018) ::
__Armaghane Danesh__ 2018, 23(3): 0-0 Back to browse issues page
In vitro cytotoxic effects of Melatonin on the chronic myeloid leukemia cell line K562
Meysam Abtahi Foroushani PhD 1, Masumeh Ahanjan 2
1- Associate Professor , meysamabtahi@hotmail.com
2- Msc
Abstract:   (140 Views)
Background and Aim: Melatonin is produced by various tissues in the body. The oncostatic properties of melatonin has been documented in some previous study. This study was designed to investigate the effects of melatonin on K562 cell line as a model of chronic myeloid leukemia (CML).
Methods: In this experimental trial, 104 × 6 cells of K562 were cultured in the presence of 0, 50, 75, 100, 150, 200 µM concentrations of melatonin for 24 hours. The morphological changes of the treated cells were evaluated using an inverse optical microscope as compared to the control sample. The effects of inhibitory growth and cytotoxicity of melatonin were measured using MTT (3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide) and Neutral Red. Differentiation was evaluated by Wright-Giemsa staining. Data analysis was performed using SPSS software and Kruskal-Wallis test (P<0.05).
 
Results: Analysis of the results showed a significant difference between the concentrations of 0 and 50 µM with other concentrations and 150 and 200 µM concentrations with other concentrations in this cell line. The highest inhibitory effect of cell growth was observed in high concentrations of melatonin. The maximum cytotoxic effect of melatonin on the K562 is at a concentration of 200 µM. While at 50 µM the effect of cytotoxicity of melatonin was minimal (P<0.05).
Conclusion: Melatonin can regress the proliferation of K562 cancer cell line. Therefore, melatonin may be added to the CML treatment protocol. Nevertheless, this study is a preliminary in vitro survey.
Keywords: Cytotoxic effects, Leukemia, Melatonin, K562.
     
Type of Study: Research | Subject: Immunology
Received: 2018/03/11 | Accepted: 2018/07/25 | Published: 2018/08/3
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Abtahi Foroushani M, ahanjan M. In vitro cytotoxic effects of Melatonin on the chronic myeloid leukemia cell line K562. Armaghane danesh. 2018; 23 (3)
URL: http://armaghanj.yums.ac.ir/article-1-2139-en.html


Volume 23, Issue 3 (8-2018) Back to browse issues page
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